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Leuk Res. 2009 Oct;33(10):1405-8. doi: 10.1016/j.leukres.2009.04.008. Epub 2009 May 2.

Characterization of a new myeloid leukemia cell line with normal cytogenetics (CG-SH).

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  • 1Department of Medicine, the Louisiana State University Health Sciences Center, Shreveport, LA 71130, USA. rmunker@lsuhsc.edu

Abstract

A new myeloid leukemia cell line (CG-SH) with normal cytogenetics was established from a patient with acute myelogenous leukemia (AML) following myelodysplastic syndrome (MDS). The cells of CG-SH are immature blasts and have an immature myeloid phenotype (positive for myeloperoxidase, CD7, CD34, CD38, CD117, HLA-DR, negative for CD10, CD19, CD20, CD41, CD42). A partial expression of CD13, CD15, CD65 and a weak expression of CD33 and CD133 was noted. The cells are negative for EBER. By molecular analysis, a mutation of NRAS and heterozygous mutations of RUNX1 were detected. No mutations were detected in FLT3-ITD, MLL-PTD or NPM1. By real-time PCR, a series of 19 microRNAs was identified which are strongly expressed in CG-SH. In conclusion, a new cell line was established which will be useful for the study of AML with normal cytogenetics and mutations in NRAS and/or RUNX1.

PMID:
19414191
[PubMed - indexed for MEDLINE]
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