Display Settings:

Format

Send to:

Choose Destination
We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
J Biomed Opt. 2009 Mar-Apr;14(2):024012. doi: 10.1117/1.3088140.

Quantitation and mapping of tissue optical properties using modulated imaging.

Author information

  • 1Modulated Imaging, Inc., 1002 Health Sciences Road, Irvine, California 92612, USA. david.cuccia@modulatedimaging.com

Abstract

We describe the development of a rapid, noncontact imaging method, modulated imaging (MI), for quantitative, wide-field characterization of optical absorption and scattering properties of turbid media. MI utilizes principles of frequency-domain sampling and model-based analysis of the spatial modulation transfer function (s-MTF). We present and compare analytic diffusion and probabilistic Monte Carlo models of diffuse reflectance in the spatial frequency domain. Next, we perform MI measurements on tissue-simulating phantoms exhibiting a wide range of l values (0.5 mm to 3 mm) and (micro(s) (')micro(a)) ratios (8 to 500), reporting an overall accuracy of approximately 6% and 3% in absorption and reduced scattering parameters, respectively. Sampling of only two spatial frequencies, achieved with only three camera images, is found to be sufficient for accurate determination of the optical properties. We then perform MI measurements in an in vivo tissue system, demonstrating spatial mapping of the absorption and scattering optical contrast in a human forearm and dynamic measurements of a forearm during venous occlusion. Last, metrics of spatial resolution are assessed through both simulations and measurements of spatially heterogeneous phantoms.

PMID:
19405742
[PubMed - indexed for MEDLINE]
PMCID:
PMC2868524
Free PMC Article

Images from this publication.See all images (13)Free text

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7
Fig. 8
Fig. 9
Fig. 10
Fig. 11
Fig. 12
Fig. 13
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Icon for Silverchair Information Systems Icon for PubMed Central
    Loading ...
    Write to the Help Desk