J Chromatogr. 1991 Sep 13;569(1-2):243-60.

Sodium dodecyl sulphate electrophoresis of urinary proteins.

Bianchi-Bosisio A, D'Agrosa F, Gaboardi F, Gianazza E, Righetti PG.

Source

Clinical Chemistry Laboratory, Ospedale A. Uboldo, Milan, Italy.

Abstract

The analysis of urinary proteins and their identification are discussed, particularly in regard to the technique of sodium dodecyl sulphate electrophoresis in polyacrylamide gradient gels. Urine collection, storage and preparation are evaluated, especially in regard to problems connected with concentration and dialysis of such samples. The instrumental approach to sodium dodecyl sulphate polyacrylamide gel electrophoresis represented by the Phast System appears to be particularly valuable in routine clinical analysis of urine specimens, since no sample pretreatment is required. The following types of proteinurias are evaluated: (a) orthostatic proteinurias; (b) post-renal proteinurias; (c) Bence-Jones proteinuria; (d) lower and upper urinary tract infection (cystitis and pyelonephritis) and (e) diabetes mellitus proteinurias.

PMID:: 1939488; [PubMed - indexed for MEDLINE]

Publication Types, MeSH Terms

Publication Types

MeSH Terms

Supplemental Content

Save items

Related citations in PubMed

Diagnostic significance of SDS-PAA-electrophoresis of urinary proteins: different forms of proteinuria and their correlation to renal diseases. [Curr Probl Clin Biochem. 1979]
Diagnostic significance of SDS-PAA-electrophoresis of urinary proteins: different forms of proteinuria and their correlation to renal diseases.
Boesken WH. Curr Probl Clin Biochem. 1979; (9):235-48.
Analysis of proteinuria using a commercial system for automated electrophoresis and isoelectric focusing. [Ann Clin Biochem. 1988]
Analysis of proteinuria using a commercial system for automated electrophoresis and isoelectric focusing.
Jackson PJ, Sampson CJ, Cooper EH, Heney D, Brocklebank JT. Ann Clin Biochem. 1988 May; 25 ( Pt 3):319-24.
Routine diagnosis with PhastSystem compared to conventional electrophoresis: automated sodium dodecyl sulfate-polyacrylamide gel electrophoresis, silver staining and western blotting of urinary proteins. [Electrophoresis. 1989]
Routine diagnosis with PhastSystem compared to conventional electrophoresis: automated sodium dodecyl sulfate-polyacrylamide gel electrophoresis, silver staining and western blotting of urinary proteins.
Scherberich JE, Fischer P, Bigalke A, Stangl P, Wolf GB, Haimerl M, Schoeppe W. Electrophoresis. 1989 Jan; 10(1):58-62.
Review Clinical analysis of human urinary proteins using high resolution electrophoretic methods. [Electrophoresis. 1998]
Review Clinical analysis of human urinary proteins using high resolution electrophoretic methods.
Marshall T, Williams KM. Electrophoresis. 1998 Jul; 19(10):1752-70.
Review Electrophoretic analysis of Bence Jones proteinuria. [Electrophoresis. 1999]
Review Electrophoretic analysis of Bence Jones proteinuria.
Marshall T, Williams KM. Electrophoresis. 1999 Jun; 20(7):1307-24.

See reviews... See all...

Cited by 2 PubMed Central articles

Identification of genes up-regulated in urothelial tumors: the 67-kd laminin receptor and tumor-associated trypsin inhibitor. [Am J Pathol. 2003]
Identification of genes up-regulated in urothelial tumors: the 67-kd laminin receptor and tumor-associated trypsin inhibitor.
Diggle CP, Cruickshank S, Olsburgh JD, Pellegrin S, Smith B, Banks RE, Selby PJ, Knowles MA, Southgate J, Harnden P. Am J Pathol. 2003 Aug; 163(2):493-504.
Molecular cloning and characterization of a novel carboxylesterase-like protein that is physiologically present at high concentrations in the urine of domestic cats (Felis catus). [Biochem J. 2003]
Molecular cloning and characterization of a novel carboxylesterase-like protein that is physiologically present at high concentrations in the urine of domestic cats (Felis catus).
Miyazaki M, Kamiie K, Soeta S, Taira H, Yamashita T. Biochem J. 2003 Feb 15; 370(Pt 1):101-10.

Related information

Related Citations
Calculated set of PubMed citations closely related to the selected article(s) retrieved using a word weight algorithm. Related articles are displayed in ranked order from most to least relevant, with the “linked from” citation displayed first.
Compound
PubChem chemical compound records that cite the current articles. These references are taken from those provided on submitted PubChem chemical substance records. Multiple substance records may contribute to the PubChem compound record.
Substance
PubChem chemical substance records that cite the current articles. These references are taken from those provided on submitted PubChem chemical substance records.
Cited in PMC
Full-text articles in the PubMed Central Database that cite the current articles.

Recent activity

Clear Turn Off Turn On

Sodium dodecyl sulphate electrophoresis of urinary proteins.
Sodium dodecyl sulphate electrophoresis of urinary proteins.
J Chromatogr. 1991 Sep 13 ;569(1-2):243-60.

PubMed
Bone marrow laser revascularisation for treating refractory angina due to diffus...
Bone marrow laser revascularisation for treating refractory angina due to diffuse coronary heart disease.
Eur J Cardiothorac Surg. 2009 Jul ;36(1):192-4. Epub 2009 Apr 25 .

PubMed
Effects of NO/L-arginine pathway on gallbladder contractility in bile duct ligat...
Effects of NO/L-arginine pathway on gallbladder contractility in bile duct ligated guinea pigs.
J Surg Res. 2009 Jul ;155(1):70-6. Epub 2008 Sep 4 .

PubMed
5-Methylcytosine content of DNA in blood, synovial mononuclear cells and synovia...
5-Methylcytosine content of DNA in blood, synovial mononuclear cells and synovial tissue from patients affected by autoimmune rheumatic diseases.
J Chromatogr. 1991 May 31 ;566(2):481-91.

PubMed
Chlorhexidine and evidence-based labor management.
Chlorhexidine and evidence-based labor management.
Am J Obstet Gynecol. 2009 Aug ;201(2):e16-7; author reply e17. Epub 2009 Apr 26 .

PubMed

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

PubMed

Result Filters

Display Settings:

Send to:

Sodium dodecyl sulphate electrophoresis of urinary proteins.

Source

Abstract

Publication Types, MeSH Terms

Publication Types

MeSH Terms

Supplemental Content

Save items

Related citations in PubMed

Cited by 2 PubMed Central articles

Related information

Recent activity

Simple NCBI Directory

Getting Started

Resources

Popular

Featured

NCBI Information