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Clin Lab Med. 2009 Mar;29(1):15-29. doi: 10.1016/j.cll.2009.01.001.

Microarrays in glycoproteomics research.

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  • 1Van Andel Research Institute, 333 Bostwick NE, Grand Rapids, MI 49503, USA.

Abstract

Microarrays have been extremely useful for investigating binding interactions among diverse types of molecular species, with the main advantage being the ability to examine many interactions using small amounts of samples and reagents. Microarrays are increasingly being used to advance research in the field of glycobiology. Several types of microarrays are being used in the study of glycans and proteins in glycobiology, including glycan arrays to study the recognition of carbohydrates, lectin arrays to determine carbohydrate expression on purified proteins or on cells, and antibody arrays to examine the variation in particular glycan structures on specific proteins. This article covers the technology and applications of these types of microarrays, and their use for obtaining complementary information on various aspects of glycobiology.

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