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J Occup Med Toxicol. 2009 Apr 22;4:8. doi: 10.1186/1745-6673-4-8.

High abundances of neurotrophin 3 in atopic dermatitis mast cell.

Author information

  • 1Institute of Occupational Medicine, Charité - Universitätsmedizin Berlin, Free University and Humboldt University, D-14195 Berlin, Germany. david.quarcoo@charite.de.

Abstract

BACKGROUND:

Neurotrophin 3 (NT-3) is a member of the neurotrophin family, a group of related proteins that are known to regulate neuro-immune interactions in allergic diseases. Their cellular sources and role in the recruitment of mast cell precursors in atopic dermatitis have not been characterized in detail so far.

OBJECTIVE:

Characterize NT-3 on a transcriptional and translational level in individuals with atopic dermatitis with special focus on mast cells.

METHODS:

To meet this objective NT-3 levels in the serum of AD patients were measured, the effect of NT-3 on keratinocytes was evaluated and the gene expression and regulation assessed using ELISA, immunohistochemistry and RNA quantification.

RESULTS:

Systemic levels of NT-3 were found to be higher in individuals with AD as compared to healthy controls. A distinct genetic expression was found in the various cells of the skin. In lesional mast cells of individuals with atopic dermatitis an increased amount of NT-3 was apparent. Functional in vitro experiments demonstrated that NT-3 stimulation led to a suppression of IL-8 secretion by HaCat cells.

CONCLUSION:

These findings could imply a role for NT-3 in the pathogenesis of allergic skin diseases.

PMID:
19386090
[PubMed]
PMCID:
PMC2680865
Free PMC Article
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