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J Bacteriol. 2009 Jun;191(12):4025-9. doi: 10.1128/JB.00118-09. Epub 2009 Apr 17.

Genomic sequencing reveals regulatory mutations and recombinational events in the widely used MC4100 lineage of Escherichia coli K-12.

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  • 1School of Molecular and Microbial Biosciences, University of Sydney, Sydney, New South Wales, Australia.

Abstract

The genome of an Escherichia coli MC4100 strain with a lambda placMu50 fusion revealed numerous regulatory differences from MG1655, including one that arose during laboratory storage. The 194 mutational differences between MC4100(MuLac) and other K-12 sequences were mostly allocated to specific lineages, indicating the considerable mutational divergence between K-12 strains.

PMID:
19376874
[PubMed - indexed for MEDLINE]
PMCID:
PMC2698400
Free PMC Article

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