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Zebrafish. 2009 Mar;6(1):69-77. doi: 10.1089/zeb.2008.0555.

A primer for morpholino use in zebrafish.

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  • 1Department of Genetics, Cell Biology, and Development, University of Minnesota, Minneapolis, Minnesota, USA.

Abstract

Morpholino oligonucleotides are the most common anti-sense "knockdown" technique used in zebrafish (Danio rerio). This review discusses common practices for the design, preparation, and deployment of morpholinos in this vertebrate model system. Off-targeting effects of morpholinos are discussed as well as method to minimize this potentially confounding variable via co-injection of a tP53-targeting morpholino. Finally, new uses of morpholinos are summarized and contextualized with respect to the complementary, DNA-based knockout technologies recently developed for zebrafish.

PMID:
19374550
[PubMed - indexed for MEDLINE]
PMCID:
PMC2776066
Free PMC Article
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