Send to:

Choose Destination
See comment in PubMed Commons below
J Proteome Res. 2008 Dec;7(12):5167-76.

Phosphorylation analysis of primary human T lymphocytes using sequential IMAC and titanium oxide enrichment.

Author information

  • 1CSIC/UAB Proteomics Laboratory, IIBB-CSIC, IDIBAPS, Facultad de Medicina, Campus UAB, 08193 Bellaterra, Spain.


T lymphocytes mediate cellular and humoral defense against foreign bodies or autoantigens. An understanding of T-cell information processing furthers studies of the immunological response. We describe a large-scale phosphorylation analysis of primary T cells using a multidimensional separation strategy, involving preparative SDS-PAGE for prefractionation, in-gel digestion and sequential phosphopeptide enrichment using IMAC and TiO2. A total of 281 phosphorylation sites (197 of high confidence, Ascore > 15), mapping to 204 human gene sequences, were identified by LC-MS(n) analysis in an LTQ linear ion trap. Subsequently, we created the LymPHOS database (, which links mass spectrometric peptide information to phosphorylation sites and phosphoprotein sequences.

[PubMed - indexed for MEDLINE]
PubMed Commons home

PubMed Commons

How to join PubMed Commons

    Supplemental Content

    Loading ...
    Write to the Help Desk