GC-1 mRHBDD1 knockdown spermatogonia cells lose their spermatogenic capacity in mouse seminiferous tubules

Yong Wang; Wei Song; Shuchun Li; Xin Guan; Shiying Miao; Shudong Zong; S S Koide; Linfang Wang

doi:10.1186/1471-2121-10-25

GC-1 mRHBDD1 knockdown spermatogonia cells lose their spermatogenic capacity in mouse seminiferous tubules

BMC Cell Biol. 2009 Apr 10:10:25. doi: 10.1186/1471-2121-10-25.

Authors

Yong Wang¹, Wei Song, Shuchun Li, Xin Guan, Shiying Miao, Shudong Zong, S S Koide, Linfang Wang

Affiliation

¹ State Key Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Peking Union Medical College, Tsinghua University 5 Dong Dan San Tiao, Beijing 100005, PR China. wang.linfang@imicams.ac.cn

Abstract

Background: Apoptosis is important for regulating spermatogenesis. The protein mRHBDD1 (mouse homolog of human RHBDD1)/rRHBDD1 (rat homolog of human RHBDD1) is highly expressed in the testis and is involved in apoptosis of spermatogonia. GC-1, a spermatogonia cell line, has the capacity to differentiate into spermatids within the seminiferous tubules. We constructed mRHBDD1 knockdown GC-1 cells and evaluated their capacity to differentiate into spermatids in mouse seminiferous tubules.

Results: Stable mRHBDD1 knockdown GC-1 cells were sensitive to apoptotic stimuli, PS341 and UV irradiation. In vitro, they survived and proliferated normally. However, they lost the ability to survive and differentiate in mouse seminiferous tubules.

Conclusion: Our findings suggest that mRHBDD1 may be associated with mammalian spermatogenesis.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Apoptosis / drug effects
Apoptosis / radiation effects
Blotting, Western
Boronic Acids / pharmacology
Bortezomib
Cell Line
Cell Proliferation
ErbB Receptors / genetics
ErbB Receptors / metabolism
ErbB Receptors / physiology*
Flow Cytometry
Gene Expression Profiling
Gene Knockdown Techniques
Green Fluorescent Proteins / genetics
Green Fluorescent Proteins / metabolism
Male
Membrane Proteins
Mice
Mice, Inbred BALB C
Pyrazines / pharmacology
RNA, Small Interfering / genetics
Recombinant Fusion Proteins / genetics
Recombinant Fusion Proteins / metabolism
Regeneration
Reverse Transcriptase Polymerase Chain Reaction
Seminiferous Tubules / cytology
Seminiferous Tubules / physiology*
Seminiferous Tubules / surgery
Spermatogenesis / genetics
Spermatogenesis / physiology*
Spermatogonia / cytology*
Spermatogonia / metabolism
Spermatogonia / transplantation
Testis / cytology
Testis / metabolism
Transfection
Ultraviolet Rays

Substances

Boronic Acids
Membrane Proteins
Pyrazines
RNA, Small Interfering
Recombinant Fusion Proteins
Rhbdf1 protein, mouse
Green Fluorescent Proteins
Bortezomib
ErbB Receptors