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Neurosci Lett. 2009 Mar 20;452(3):238-40. doi: 10.1016/j.neulet.2009.01.025. Epub 2009 Jan 14.

Adipose stromal cells-conditional medium protected glutamate-induced CGNs neuronal death by BDNF.

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  • 1Department of Neurology, School of Medicine, Indiana University, Indianapolis, IN 46202, USA.

Abstract

The delivery of factors secreted by adipose stromal cells (ASC) to the brain may be a viable neuroprotective therapeutic option. In this study, we investigated the bioactivity of ASC-conditioned medium (ASC-CM) in glutamate-induced neurotoxicity and found that the ASC-CM significantly blocked glutamate neurotoxicity. We identified the brain-derived neurotrophic factor (BDNF) in the ASC-CM by using Western blot and demonstrated that this activity was critical for the neuroprotective effect of ASC-CM in excytotoxicity models. Furthermore, inactivating BDNF also attenuated the suppression by ASC-CM of glutamate-induced caspase-3 activity, but not p38 phosphorylation. These findings suggest that among ASC secrete a potent combination of factors, BDNF play a major role in neuroprotection against excytotoxicity.

PMID:
19348731
[PubMed - indexed for MEDLINE]
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