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J Chromatogr A. 2009 May 15;1216(20):4457-64. doi: 10.1016/j.chroma.2009.03.041. Epub 2009 Mar 20.

Automated sample preparation method for mass spectrometry analysis on recombinant proteins.

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  • 1School of Biotechnology, Department of Proteomics, AlbaNova University Center, Royal Institute of Technology, 106 91 Stockholm, Sweden.

Abstract

A completely automated procedure for the purification and desalting of proteins with a polyhistidine purification tag prior to mass spectrometry analysis is presented. The system is ideal for rapid quality control and optimization studies and it provides researchers with a straightforward, reliable tool for studies of recombinant proteins. Forty-eight samples can be prepared within 4.5h and only small cultivation and buffer volumes are needed. In this proof of concept, 19,000-35,000Da recombinant proteins from both crude and clarified cell lysates were successfully prepared for subsequent analysis by electrospray ionization and matrix-assisted laser desorption/ionization mass spectrometry as well as by gel electrophoresis.

PMID:
19345368
[PubMed - indexed for MEDLINE]
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