Display Settings:

Format

Send to:

Choose Destination
See comment in PubMed Commons below
Curr Biol. 2009 Apr 28;19(8):630-9. doi: 10.1016/j.cub.2009.02.047. Epub 2009 Apr 2.

Tdrd6 is required for spermiogenesis, chromatoid body architecture, and regulation of miRNA expression.

Author information

  • 1Department of Gene and Cell Medicine, Mount Sinai School of Medicine, New York, NY 10029, USA.

Abstract

BACKGROUND:

Chromatoid bodies (CBs) are characteristic spermatid organelles, which were suggested to function in RNA storage and small RNA processing but whose functions remain largely unknown. CB components include Mili, Miwi, and Tudor domain proteins such as Tdrd6, whose contribution to CB structure and function is elusive.

RESULTS:

We determined gametogenesis stage- and male-specific expression and localization of Tdrd6, identified a C-terminally truncated form as predominant after meiosis I, and demonstrated direct physical interaction of Tdrd6 with the CB components Mili and Miwi. Development from round into elongated spermatids is abrogated in Tdrd6(-/-) mice. Their round spermatids bear "ghost" CBs, whose architecture is greatly disrupted. Mael, Miwi, and Mvh do not localize to the Tdrd6-deficient CBs, but retrotransposons are not significantly activated. However, more than 50 miRNAs are more abundant in Tdrd6(-/-) testes, as are exemplary pre- and pri-miRNAs.

CONCLUSION:

We conclude that Tdrd6 is essential for spermiogenesis, for CB structure, and for proper mature and precursor miRNA expression.

PMID:
19345099
[PubMed - indexed for MEDLINE]
PMCID:
PMC2719840
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for PubMed Central
    Loading ...
    Write to the Help Desk