Angew Chem Int Ed Engl. 2009;48(18):3362-5.

A single residue influences the reaction mechanism of ammonia lyases and mutases.

Source

Institute of Biochemistry, Dept. of Biotechnology & Enzyme Catalysis, Greifswald University, Felix-Hausdorff-Strasse 4, 17487 Greifswald, Germany.

Erratum in

Angew Chem Int Ed Engl. 2010 May 25;49(23):3860.

Abstract

All ways lead to Rome? Computer modeling and kinetic measurements identified a distinct residue in Phe/Tyr ammonia lyases (PAL/TAL) which controls whether the Friedel-Crafts or an E(1)cB reaction mechanism takes place. Hence, Glu484 in pcPAL favors the Friedel-Crafts reaction (see picture, MIO = 4-methylidene imidazol-5-one) whereas an Asn in TAL gives an elimination reaction. These mechanistic investigations also reveal activity of a PAL mutant and a TAL towards an amino alcohol.

PMID:: 19343746; [PubMed - indexed for MEDLINE]

Publication Types, MeSH Terms, Substances

Publication Types

Research Support, Non-U.S. Gov't

MeSH Terms

Substances

LinkOut - more resources

Full Text Sources

Molecular Biology Databases

GLUTAMIC ACID HYDROCHLORIDE - HSDB

Supplemental Content

Save items

Related citations in PubMed

Discovery of a substrate selectivity switch in tyrosine ammonia-lyase, a member of the aromatic amino acid lyase family. [Chem Biol. 2006]
Discovery of a substrate selectivity switch in tyrosine ammonia-lyase, a member of the aromatic amino acid lyase family.
Watts KT, Mijts BN, Lee PC, Manning AJ, Schmidt-Dannert C. Chem Biol. 2006 Dec; 13(12):1317-26.
Preparation of unnatural amino acids with ammonia-lyases and 2,3-aminomutases. [Methods Mol Biol. 2012]
Preparation of unnatural amino acids with ammonia-lyases and 2,3-aminomutases.
Poppe L, Paizs C, Kovács K, Irimie FD, Vértessy B. Methods Mol Biol. 2012; 794:3-19.
Structural determinants and modulation of substrate specificity in phenylalanine-tyrosine ammonia-lyases. [Chem Biol. 2006]
Structural determinants and modulation of substrate specificity in phenylalanine-tyrosine ammonia-lyases.
Louie GV, Bowman ME, Moffitt MC, Baiga TJ, Moore BS, Noel JP. Chem Biol. 2006 Dec; 13(12):1327-38.
Review Friedel-Crafts-type mechanism for the enzymatic elimination of ammonia from histidine and phenylalanine. [Angew Chem Int Ed Engl. 2005]
Review Friedel-Crafts-type mechanism for the enzymatic elimination of ammonia from histidine and phenylalanine.
Poppe L, Rétey J. Angew Chem Int Ed Engl. 2005 Jun 13; 44(24):3668-88.
Review Ammonia lyases and aminomutases as biocatalysts for the synthesis of α-amino and β-amino acids. [Curr Opin Chem Biol. 2011]
Review Ammonia lyases and aminomutases as biocatalysts for the synthesis of α-amino and β-amino acids.
Turner NJ. Curr Opin Chem Biol. 2011 Apr; 15(2):234-40. Epub 2010 Dec 3.

See reviews... See all...

Related information

Related Citations
Calculated set of PubMed citations closely related to the selected article(s) retrieved using a word weight algorithm. Related articles are displayed in ranked order from most to least relevant, with the “linked from” citation displayed first.
Compound (MeSH Keyword)
PubChem chemical compound records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.
Substance (MeSH Keyword)
PubChem chemical substance (submitted) records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.

Recent activity

Clear Turn Off Turn On

A single residue influences the reaction mechanism of ammonia lyases and mutases...
A single residue influences the reaction mechanism of ammonia lyases and mutases.
Angew Chem Int Ed Engl. 2009 ;48(18):3362-5.

PubMed
Endothelial dysfunction of the coronary microvasculature is associated with coro...
Endothelial dysfunction of the coronary microvasculature is associated with coronary blood flow regulation in patients with early atherosclerosis.
Circulation. 1991 Nov ;84(5):1984-92.

PubMed
Psychological factors related to prehospital delay during acute myocardial infar...
Psychological factors related to prehospital delay during acute myocardial infarction.
Circulation. 1991 Nov ;84(5):1969-76.

PubMed
A proteomic-based approach for the identification of immunodominant Cryptococcus...
A proteomic-based approach for the identification of immunodominant Cryptococcus neoformans proteins.
Proteomics. 2009 May ;9(9):2578-88.

PubMed
Biophysical response to pulsed laser microbeam-induced cell lysis and molecular ...
Biophysical response to pulsed laser microbeam-induced cell lysis and molecular delivery.
J Biophotonics. 2008 Mar ;1(1):24-35.

PubMed

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

PubMed

Result Filters

Display Settings:

Send to: