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Appl Opt. 2009 Apr 1;48(10):D109-20.

Validation of an integrated Raman- and angular-scattering microscopy system on heterogeneous bead mixtures and single human immune cells.

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  • 1Institute of Optics, University of Rochester, Rochester, New York 14627, USA.


A microscopy system has been constructed that is capable of simultaneously acquiring both Raman spectra and angle-resolved elastic-scattering patterns in either epi- or transillumination modes with a 7 mum spot size. The benefits and drawbacks of the epi- and transillumination modalities are discussed. Validation studies have been performed on single beads of a few micrometers in size, as well as on ensembles of submicrometer particles. In addition, transilluminated Raman and elastic-scattering spectra were obtained from single granulocytes and peripheral blood monocytes. Both the Raman- and the elastic-scattering channels show clear differences between the two types of immune cells.

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