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Int J Mol Sci. 2008 Dec;9(12):2601-13. doi: 10.3390/ijms9122601. Epub 2008 Dec 11.

Tobacco OPBP1 enhances salt tolerance and disease resistance of transgenic rice.

Author information

  • 1Department of Plant Pathology, China Agricultural University, Beijing, China. chenxj@cau.edu.cn

Abstract

Osmotin promoter binding protein 1 (OPBP1), an AP2/ERF transcription factor of tobacco, has been demonstrated to function in disease resistance and salt tolerance in tobacco. To increase stress tolerant capability of rice, we generated rice plants with an OPBP1 overexpressing construct. Salinity shock treatment with 250 mM NaCl indicated that most of the OPBP1 transgenic plants can survive, whereas the control seedlings cannot. Similar recovery was found by using the seedlings grown in 200 mM NaCl for two weeks. The OPBP1 transgenic and control plants were also studied for oxidative stress tolerance by treatment with paraquat, showing the transgenic lines were damaged less in comparison with the control plants. Further, the OPBP1 overexpression lines exhibited enhanced resistance to infections of Magnaporthe oryzae and Rhizoctonia solani pathogens. Gene expressing analysis showed increase in mRNA accumulation of several stress related genes. These results suggest that expression of OPBP1 gene increase the detoxification capability of rice.

KEYWORDS:

AP2/EREBP; disease resistance; salt tolerance; transcription factor; transgenic rice

PMID:
19330095
[PubMed]
PMCID:
PMC2635653
Free PMC Article
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