Anti-inflammatory activity of (-)-aptosimon isolated from Daphne genkwa in RAW264.7 cells

Mee-Young Lee; Bo-Young Park; Ok-Kyoung Kwon; Ji-Eun Yuk; Sei-Ryang Oh; Hui-Seong Kim; Hyeong-Kyu Lee; Kyung-Seop Ahn

doi:10.1016/j.intimp.2009.03.012

Anti-inflammatory activity of (-)-aptosimon isolated from Daphne genkwa in RAW264.7 cells

Int Immunopharmacol. 2009 Jul;9(7-8):878-85. doi: 10.1016/j.intimp.2009.03.012. Epub 2009 Mar 26.

Authors

Mee-Young Lee¹, Bo-Young Park, Ok-Kyoung Kwon, Ji-Eun Yuk, Sei-Ryang Oh, Hui-Seong Kim, Hyeong-Kyu Lee, Kyung-Seop Ahn

Affiliation

¹ Immune Modulator Research Center, KRIBB, 111 Gwahangno, Yuseong-gu, Daejeon 305-806, South Korea.

PMID: 19328870
DOI: 10.1016/j.intimp.2009.03.012

Abstract

In the present study, we investigated that (-)-aptosimon, isolated from flower buds of Daphne genkwa, inhibited cyclooxygenase-2 (COX-2) and inducible nitric oxide (NO) synthase (iNOS) expression in lipopolysaccharide (LPS)-stimulated RAW264.7 cells. Similarly, (-)-aptosimon suppressed tumor necrosis factor (TNF)-alpha production. Our results clearly indicated that (-)-aptosimon inhibited LPS-induced nuclear factor-kappaB (NF-kappaB) activation, by preventing degradation of the inhibitor kappa B-alpha (IkappaB-alpha). (-)-Aptosimon also inhibited interleukin-4 (IL-4) and interleukin-13 (IL-13) production in ConA-induced splenocytes. In conclusion, the anti-inflammatory effects of (-)-aptosimon are attributed to the suppression of pro-inflammatory cytokines and mediators by blocking NF-kappaB activation. These data suggest that (-)-aptosimon as a potential therapeutic agent for inflammation-associated disorders.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Anti-Inflammatory Agents, Non-Steroidal / pharmacology*
Cell Line
Concanavalin A / metabolism
Cyclooxygenase 2 / genetics
Cyclooxygenase 2 / metabolism
Daphne*
Dioxoles / pharmacology*
Enzyme Activation / drug effects
Enzyme Activation / immunology
Flowers
Furans / pharmacology*
Interleukin-13 / genetics
Interleukin-13 / metabolism
Interleukin-4 / genetics
Interleukin-4 / metabolism
Lipopolysaccharides / metabolism
Macrophages / drug effects*
Macrophages / immunology
Macrophages / metabolism
Macrophages / pathology
Mice
NF-kappa B / antagonists & inhibitors
Nitric Oxide Synthase Type II / genetics
Nitric Oxide Synthase Type II / metabolism
Phytotherapy / trends*
Transcriptional Activation / drug effects
Tumor Necrosis Factor-alpha / genetics
Tumor Necrosis Factor-alpha / metabolism

Substances

Anti-Inflammatory Agents, Non-Steroidal
Dioxoles
Furans
Interleukin-13
Lipopolysaccharides
NF-kappa B
Tumor Necrosis Factor-alpha
Concanavalin A
Interleukin-4
aptosimon
Nitric Oxide Synthase Type II
Nos2 protein, mouse
Ptgs2 protein, mouse
Cyclooxygenase 2