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J Immunol Methods. 2009 May 15;344(1):58-63. doi: 10.1016/j.jim.2009.03.007. Epub 2009 Mar 26.

A streamlined method for rapid and sensitive chromatin immunoprecipitation.

Author information

  • 1Department of Microbiology, North Carolina State University, Raleigh, NC 27695, USA. mlsikes@ncsu.edu

Abstract

We report a streamlined procedure to efficiently carry samples from chromatin to qPCR-compatible DNA in as little as 4 h. We use this streamlined ChIP to quantify histone H3 modifications at active (cad) and repressed (T early alpha) promoters in a Rag1-deficient pro-T cell line after 1-2 h IP. We further show that the protocol readily quantified histone modifications in chromatin from 10(4) Rag-deficient DN thymocytes. Taken together, these data outline a simple, cost-effective procedure for efficient ChIP analysis.

PMID:
19328803
[PubMed - indexed for MEDLINE]
PMCID:
PMC2692951
Free PMC Article

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