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Clin Chem. 2009 Jun;55(6):1061-6. doi: 10.1373/clinchem.2007.100008. Epub 2009 Mar 26.

Steroid hormone analysis by tandem mass spectrometry.

Author information

  • 1Department of Medicine, Georgetown University, Washington, DC 20007, USA. sjs44@georgetown.edu

Abstract

BACKGROUND:

New high-performance liquid chromatography/tandem mass spectrometry (LC-MS/MS) methods are among the most successful approaches to improve specificity problems inherent in many immunoassays.

CONTENT:

We emphasize problems with immunoassays for the measurement of steroids and review the emerging role of LC-MS/MS in the measurement of clinically relevant steroids. The latest generation of tandem mass spectrometers has superior limits of quantification, permitting omission of previously employed derivatization steps. The measurement of steroid profiles in the diagnosis and treatment of congenital adrenal hyperplasia, adrenal insufficiency, chronic pelvic pain and prostatitis, oncology (breast cancer), and athletes has important new applications.

CONCLUSIONS:

LC-MS/MS now affords the specificity, imprecision, and limits of quantification necessary for the reliable measurement of steroids in human fluids, enhancing diagnostic capabilities, particularly when steroid profiles are available.

PMID:
19325015
[PubMed - indexed for MEDLINE]
PMCID:
PMC3634331
Free PMC Article
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