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Biotechniques. 1991 Aug;11(2):166-8, 170-1.

Detection of loss of heterozygosity in tumor DNA samples by PCR.

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  • 1Oncogenetic Section, NCI, NIH, Bethesda, MD 20892.


We demonstrate that PCR amplification of human genomic DNA can be used for the detection of loss of heterozygosity (LOH) in tumor samples. A 250-bp fragment containing codon 72 of the human p53 gene was amplified, ThaI digested and electrophoresed. Tumor LOH is detectable both by ethidium bromide staining and autoradiography, despite 25% contamination with normal DNA. This technique provides a fast and reproducible alternative to conventional Southern blotting and has minimal sample requirements.

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