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Exp Parasitol. 2009 Jun;122(2):134-9. doi: 10.1016/j.exppara.2009.02.012. Epub 2009 Mar 20.

Leishmania (Viannia) panamensis: an in vitro assay using the expression of GFP for screening of antileishmanial drug.

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  • 1Programa de Estudio y Control de Enfermedades Tropicales-PECET, Universidad de Antioquia, Calle 62 No 52-59, Lab. 632, Medellín, Colombia.

Abstract

Promastigotes of Leishmania (Viannia) panamensis were successfully transfected with p6.5-egfp to express green fluorescent protein. The transfectants remained infective to macrophages, providing an in vitro model for screening antileishmanial drugs. This was demonstrated by flow cytometry of macrophage-associated GFP after exposure of infected cultures to known antileishmanial drugs, i.e. amphotericin B and glucantime. Fluorescence of GFP diminished progressively from infected cells with increasing drug concentrations used in both cases. The availability of this fluorescent assay for infection of macrophages by L. (V.) panamensis facilitates drug discovery program for the Viannia species, which differ significantly from those of the Leishmania subgenus.

PMID:
19303871
[PubMed - indexed for MEDLINE]
PMCID:
PMC3640980
Free PMC Article
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