(A) DISC1 knockdown cells exhibit cell positioning defects in utero. Control or DISC1 shRNA constructs were electroporated into E13 embryonic mouse brains and the mice were sacrificed at E15. The percentage of GFP cells in each region is shown (n=4, p<0.01). Scale bar=20 µm. CP, cortical plate; IZ, intermediate zone; VZ/SVZ, ventricular zone/subventricular zone.
(B) BrdU incorporation is reduced in DISC1 knockdown brains. Brains of mice electroporated at E13 were pulse labeled with BrdU (100 mg/kg) for 2 hours. Arrows indicate GFP and BrdU double positive cells. The bar graph shows the percentage of BrdU and GFP double positive cells to total GFP positive cells in SVZ (n=4, p<0.0005). Scale bar=20 µm.
(C) Mitotic index of DISC1 silenced cells is reduced in utero. The percentage of GFP positive cells that are also pH3 positive in the VZ is shown (n=4, p<0.01). Scale bar=20 µm. Arrowheads indicate GFP and pH3 double positive cells.
(D) DISC1 knockdown in progenitor cells causes premature cell cycle exit in utero. Control or DISC1 shRNA constructs were electroporated into E13 embryonic brains and BrdU was injected at E15. Mice were sacrificed at E16. The cell cycle exit index is measured as the percentage of the GFP-positive cells that exited the cell cycle (GFP+ BrdU+ Ki67−) divided by total GFP and BrdU double positive (GFP+ BrdU+) cells (n=5, p<0.001). Scale bar=20 µm. White arrows indicate GFP+BrdU+Ki67+ cells. Arrowheads indicate GFP+BrdU+Ki67− cells.
(E) BrdU labeling is increased in DISC1 overexpressing cells in utero. Control or WT-DISC1 plasmids were electroporated into embryonic brains at E14 and BrdU was injected 2 hours before sacrificing at E15. The percentage of GFP positive cells in the SVZ that are also BrdU positive cells is shown (n=3, * p<0.05, ***p<0.005). Scale bar=10 µm.

(A) Enhancement of TOP activity by DISC1 overexpression is abolished by β-catenin shRNAs in embryonic primary progenitors (n=6, p<0.01).
(B) Vector, WT-β-catenin, or SA-β-catenin was cotransfected with the TOP reporter into embryonic progenitors transduced with lentiviruses expressing DISC1 shRNAs. Only SA-β-catenin rescued the TOP activation defect in DISC1 knockdown cells (n=4, *p<0.05, **p<0.01).
(C) Cell positioning and BrdU incorporation defects caused by DISC1 loss-of-function are rescued by human WT-DISC1 or SA-β-catenin. The GFP positive cells that are also BrdU positive cells is shown as the percentage to control plus vector (n=5, **, p<0.01; ***, p<0.005). Scale bar=20 µm.
(D) The reduction in mitotic index caused by DISC1 knockdown in embryonic brains is rescued by human WT-DISC1 or SA-β-catenin. Arrows indicate GFP and pH3 double positive cells. The GFP positive cells that are also pH3 positive cells is shown as the percentage to control plus vector (n=4, *, p<.0.05; ***, p<0.005). Scale bar=20 µm.

(A) DISC1 interacts with endogenous GSK3β. E14 brain lysate was subjected to immunoprecipitation with anti-HA (negative control) or anti-DISC1 antibody and immunoblotted with anti-GSK3β or DISC1 antibody.
(B) GSK3β directly binds to DISC1 in vitro. Purified His-GSK3β interacts directly with DISC1 fragments 1–220aa and 356–595aa. Stars indicate the intact GST fusion proteins.
(C) GSK3β activity is reduced by DISC1 GST-fragments in vitro. Numbers in the panel indicate the relative intensity of the band compared to the no protein lane (n=3). Stars indicate the different intact GST fusion proteins.
(D) Inhibition of GSK3β activity by DISC1 peptide 1(195–238aa) is dose-dependent. The dose-response curve is shown (n=3, ***, p<0.005).
(E) Disctide-1(195–238aa) directly binds to GSK3β Surface plasmon resonance sensorgrams for GSK3β binding assays with peptide-neg, Disctide-1, L803-mts, and a peptide from CACNA1B are shown in the left panel. The arrow indicates the injection of buffer without compound. The percent theoretical maximal response is summarized in the right hand panel for these SPR experiments.
(F) Disctide2 (211–225aa) directly binds to GSK3β The schematic shows Disctide1 (mDisc aa 195–238). Each numbered line represents a 15-mer peptide (i.e. peptide2–38 – - 48) that corresponds to the DISC1 amino acids above it. The percent theoretical maximal response for SPR binding assays at 25 µM between (Disctide1 15-mers and GSK3β is summarized at the bottom of the panel. Disctide2, indicated in red, was found to bind to GSK3β.

(A) E15 embryonic brain sections were co-stained with anti-DISC1 and anti-Nestin antibody. Scale bar=20 µm.
(B) E15 embryonic brain sections were co-stained with anti-DISC1 and anti-Sox2 antibody. Scale bar=20 µm.
(C) Cell proliferation is reduced in AHP cells infected with lentivirus expressing DISC1 shRNAs. GFP expression is used as a marker for viral infection. Both DISC1 shRNAs significantly reduced cell proliferation (n=3, p<0.01).
(D) BrdU incorporation is decreased in DISC1 knockdown cells. AHPs infected with control, DISC1 shRNA-1, or shRNA-2 lentivirus were pulse labeled with 10 µM BrdU and stained with BrdU antibody. The percentage of GFP positive cells that are also BrdU positive cells is shown (n=4, p<0.01).
(E) Mitotic index is reduced in DISC1-silenced AHPs. The percentage of GFP positive cells that are also pH3 positive is shown (n=3, p<0.01).
(F) Histograms for FACS analysis of N2a cells transfected with DISC1 shRNAs. Bar graph depicts the percentage of GFP positive cells in G0/G1 (n=3, p<0.01).
(G) Cell proliferation is increased in DISC1 overexpressing cells. AHPs were infected with either control or DISC1-WT lentivirus. Cell number was counted for 2 days (n=3, p<0.01).
(H) BrdU incorporation is increased in DISC1-overexpressing AHPs. The percentage of GFP positive cells that are also BrdU positive cells is shown (n=4, p<0.001).
(I) Increased mitotic index in DISC1 overexpressing AHPs. The percentage of GFP positive cells that are also pH3 positive is shown (n=4, p<0.001).

(A) DISC1 knockdown cells exhibit proliferation defects in response to Wnt3a. Transduced AHPs were grown in medium with or without Wnt3a (200 ng/ml) and cell numbers were counted for 2 days (n=3, *p<0.05, **p<0.01).
(B) LEF/TCF activity (TOP) is decreased in DISC1-silenced primary neural progenitors. Luciferase activity with mutated LEF/TCF binding sites (FOP) is not affected by DISC1 shRNAs. The relative firefly luciferase activity normalized to Renilla luciferase activity is shown (n=6, p<0.01).
(C) TOP activity is rescued by human WT-DISC1 expression in DISC1-silenced primary neural progenitors (n=3, p<0.01).
(D) In utero TOP luciferase assay in DISC1 knockdown brains. Control or DISC1 shRNAs were coelectroporated with TOP and pRL-TK plasmids into E13 brains. Embryonic brains were harvested and subjected to luciferase assays 48 hours later. The relative TOP luciferase activity normalized to Renilla luciferase activity is shown (p<0.001).
(E) TOP activity is increased in primary neural progenitors overexpressing DISC1. Shown is the relative firefly luciferase activity normalized to Renilla luciferase activity (n=3, p<0.01).
(F) In utero TOP activity is increased with DISC1 overexpression. Luciferase activity is measured 24 hours after in utero electroporation (p<0.001).

(A) Phosphorylation of β-catenin is increased in DISC1 silenced AHPs. Bar graph shows the percentage of relative band intensity of pS33/37/T41-β-catenin compared to control (mean±S.E.M., n=3, p<0.05) .
(B) Phosphorylated β-catenin is reduced by DISC1 overexpression in AHPs. Bar graph shows the percentage of relative band intensity of pS33/37/T41-β-catenin to vector (mean±S.E.M., n=3, p<0.005) .
(C) Cyclin D1 and axin2 expression are reduced in DISC1 silenced AHPs. Bar graph shows the percentage of relative band intensity of cyclin D1 to control (mean±S.E.M., n=3, *p<0.05, **p<0.01).
(D) Cyclin D1 and axin2 expression is enhanced in DISC1 overexpressing AHPs. Bar graph shows the percentage of relative band intensity of cyclin D1 to vector (mean±S.E.M., n=3, p<0.05).
(E) GSK3β activity increases in DISC1 knockdown cells. Lysates from transduced AHPs were immunoblotted with the anti-pY216-GSK3β, pS231/234 Ngn2, or pT220/226 C/EBPα antibodies. Bar graph shows the percentage of relative band intensity of pY216-GSK3β or pS231/234 Ngn2 to control (mean±S.E.M., n=3, p<0.01).
(F) Overexpression of DISC1 suppresses GSK3β activity. Bar graph shows the percentage of relative band intensity of pY216-GSK3β or pS231/234 Ngn2to corresponding vector (mean±S.E.M., n=3, p<0.01).

(A) Control or DISC1 shRNA-1 lentivirus was stereotactically injected into adult dentate gyrus. After 4 weeks of recovery, mice received SB216763 (2 mg/kg) every other day for 2 weeks and BrdU (100 mg/kg) daily for 7 days. The GFP signal represents lentiviral infected cells in dentate gyrus. Scale bar=50 µm.
(B) DISC1 knockdown reduced adult progenitor proliferation in dentate gyrus, but this defect can be rescued by SB216763. The percentage of GFP and BrdU double positive cells is shown (n=5, *, p<.0.05; **, p<0.01; ***, p<0.005; ns, not significant). Scale bar=10 µm.
(C) DISC1 knockdown in adult mice leads to hyperlocomotion in a novel environment. DISC1 knockdown mice showed ieaterg distance traveled, movement time in a novel open field, which can be reversed by SB216763 treatment (n≥8 for each group, p<0.05).
(D) DISC1 knockdown in adult mice increased immobility in the forced swimming test, which was rescued by SB216763 (n≥8 for each group, **, p<0.01; ***, p<0.005).
(E) No behavior changes were detected in the levated plus maze test (n=15, for each group).