Determination of the 5' exon structure of the huma...[Biochem Biophys Res Commun. 1991]

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1: Biochem Biophys Res Commun. 1991 Sep 30;179(3):1255-8. Links

Determination of the 5' exon structure of the human cardiac alpha-myosin heavy chain gene.

Brand NJ, Dabhade N, Yacoub M, Barton PJ.

Department of Cardiothoracic Surgery, National Heart and Lung Institute, London, U.K.

We have deduced the exon structure of the 5' untranslated region of the human cardiac alpha-myosin heavy chain gene by cloning a cDNA for this region using the polymerase chain reaction. Comparison of the cDNA and genomic DNA sequences demonstrates that the 5' non-coding region of the alpha-myosin heavy chain gene is interrupted by two introns of 645 and 337 nucleotides. Secondly we have identified the transcriptional start-site by primer extension, corroborating the previous putative assignment for the alpha-myosin heavy chain promoter based on comparisons between the rat and human genes.

PMID: 1930170 [PubMed - indexed for MEDLINE]

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