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Biotechniques. 2009 Jan;46(1):58-60.

Genotyping of IRGM tetranucleotide promoter oligorepeats by fluorescence resonance energy transfer.

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  • 1Bernhard Nocht Institute for Tropical Medicine, Department of Molecular Medicine, Hamburg, Germany.


Fluorescence resonance energy transfer (FRET) genotyping has been well established for the rapid assessment of single nucleotide polymorphisms (SNPs) and deletions. A design is presented that allows the typing of short tandem oligo repeat sequences using the LightTyper/LightCycler system. The protocol was evaluated and applied to the typing of a tetranucleotide promoter repeat of the human gene encoding the immunity-related GTPase family, M (IRGM) molecule in >2000 individuals from Ghana, West Africa.

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