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Genesis. 2009 May;47(5):300-4. doi: 10.1002/dvg.20499.

Molecular cloning of a dominant roller mutant and establishment of DNA-mediated transformation in the nematode Pristionchus pacificus.

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  • 1Department for Evolutionary Biology, Max-Planck-Institute for Developmental Biology, Tübingen, Germany.

Abstract

We report the molecular cloning of a dominant Roller mutant of Pristionchus pacificus, which encodes a cuticle collagen. We use the mutant locus as a marker to develop transgenic technique by generating complex arrays and present flourescent-protein based transcriptional reporter constructs for P. pacificus.

2009 Wiley-Liss, Inc.

PMID:
19298013
[PubMed - indexed for MEDLINE]
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