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Inflamm Res. 2009 Sep;58(9):585-92. doi: 10.1007/s00011-009-0022-x. Epub 2009 Mar 19.

Rotavirus and coxsackievirus infection activated different profiles of toll-like receptors and chemokines in intestinal epithelial cells.

Author information

  • 1Pediatric Institute, Children's Hospital, Fudan University, 183 Fenglin Road, 200032, Shanghai, China. janexu125@hotmail.com

Abstract

OBJECTIVE:

To understand the inflammatory-immune response in intestinal epithelial cells after infection of rotavirus and coxsackievirus B3.

METHODS:

We examined by quantitative PCR the expression profiles of genes encoding five toll-like receptors (TLR) and levels of three chemokines in response to rotavirus and coxsackievirus B3 infection in a human intestinal epithelial cell line (HT-29 cells).

RESULTS:

We demonstrated that rotavirus induced significantly increased levels of mRNA expression for TLR2, TLR3, TLR7 and TLR8 in HT-29 cells in a time-dependent manner. In contrast, coxsackievirus B3 did not stimulate mRNA expression for TLR3. Rotavirus and coxsackievirus B3 also induced higher levels of mRNA expression for RANTES, IP-10 and IL-8 during the period of infection in a different manner. Finally, significantly elevated levels of RANTES, IP-10 and IL-8 were detected by ELISA in rotavirus-infected cells from 24 to 48 h.

CONCLUSION:

Our findings suggest that different patterns of TLRs and chemokines were induced in the initiation and modulation of immune response to rotavirus and coxsackievirus B3 infection.

PMID:
19296205
[PubMed - indexed for MEDLINE]
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