FEBS Lett. 2009 Apr 2;583(7):1090-5. Epub 2009 Mar 14.

Association of the eukaryotic V1VO ATPase subunits a with d and d with A.

Thaker YR, Hunke C, Yau YH, Shochat SG, Li Y, Grüber G.

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School of Biological Sciences, Nanyang Technological University, Singapore 637551, Republic of Singapore.

Abstract

Owing to the complex nature of V(1)V(O) ATPases, identification of neighboring subunits is essential for mechanistic understanding of this enzyme. Here, we describe the links between the V(1) headpiece and the V(O)-domain of the yeast V(1)V(O) ATPase via subunit A and d as well as the V(O) subunits a and d using surface plasmon resonance and fluorescence correlation spectroscopy. Binding constants of about 60 and 200 nM have been determined for the a-d and d-A assembly, respectively. The data are discussed in light of subunit a and d forming a peripheral stalk, connecting the catalytic A(3)B(3) hexamer with V(O).

PMID:: 19289121; [PubMed - indexed for MEDLINE]

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