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Nat Rev Microbiol. 2009 Apr;7(4):287-96. doi: 10.1038/nrmicro2122.

Application of 'next-generation' sequencing technologies to microbial genetics.

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  • 1The Sainsbury Laboratory, Norwich NR4 7UH, United Kingdom.


New sequencing methods generate data that can allow the assembly of microbial genome sequences in days. With such revolutionary advances in technology come new challenges in methodologies and informatics. In this article, we review the capabilities of high-throughput sequencing technologies and discuss the many options for getting useful information from the data.

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