Rapid Commun Mass Spectrom. 2009 Apr;23(8):1183-8.

A mass spectrometry based imaging method developed for the intracellular detection of HIV protease inhibitors.

Dekker LJ, van Kampen JJ, Reedijk ML, Burgers PC, Gruters RA, Osterhaus AD, Luider TM.

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Department of Neurology, Erasmus MC, Rotterdam, The Netherlands. l.dekker@erasmusmc.nl

Abstract

Mass spectrometry imaging is a promising technique for measuring drugs and drug metabolites in cells and tissues. In this manuscript we describe a method for the imaging of HIV protease inhibitors. As a model system we used Mono Mac 6 cells cultured with the HIV protease inhibitors saquinavir and nelfinavir deposited on glass slides using a cytocentrifuge. A sublimation/deposition device for homogeneous matrix deposition was constructed which allows imaging of these HIV protease inhibitors at clinically relevant concentrations. Using this matrix sublimation/deposition method, glass slides containing the cytocentrifuged cells can be measured and analyzed by two types of mass spectrometry techniques, viz. matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) and MALDI Fourier transform ion cyclotron resonance (FTICR), and this makes it possible to perform imaging rapidly (MALDI-TOF) and with a very high selectivity (MALDI-FTICR).

PMID:: 19283784; [PubMed - indexed for MEDLINE]

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