The Bloomington second chromosome deficiency kit contains a high frequency of lgl alleles. The mapped deficiencies that fail to complement the null allele lgl[4] are shown; these include deficiencies on both the left and right arms of chromosome 2. Df(2R)ST1-Df(2R)cn9 and Df(2R)P34-Df(2R)BSC26 are pairs of deficiency stocks that delete partially overlapping sequence.

The Bruinfly FRT40A collection contains a high frequency of lgl alleles. (Top) Cuticle preparations from wild-type (A), lgl[4] germline clones (B), and Kruppel-Homolog 1 (Kr-H1) (Bruinfly 13097) germline clones (C). Loss of maternal and zygotic lgl disrupts cuticle development, and the mimic of this phenotype in the Kr-H1 line is due to loss of lgl. (Bottom) Five of 24 randomly selected FRT40A-lethal P stocks, containing transposons distributed over the entirety of chromosome 2L, harbored lgl alleles.

The loss of lgl from insv mutant chromosomes is responsible for their reported multiple socket phenotypes. (A) Scanning electron micrograph of insv[23B] notum clones, which differentiate sensory organs with extra socket cells. The dotted circle highlights a four-socket organ, while the dotted square indicates an organ with two sockets and a malformed shaft. (B, B′) Cut staining in a 24-hr after puparium formation (APF) notum bearing insv[23B] clones, marked by the absence of nuclear GFP. Wild-type organs contain two large (arrowheads) and two small (arrows) Cut⁺ nuclei, while insv mutant organs contain four large Cut⁺ nuclei. (C, C′) Suppressor of Hairless [Su(H)] staining in a 24-hr APF notum bearing insv[23B] clones marked by the absence of nuclear GFP. Wild-type organs contain a single Su(H)⁺ cell (arrow), while insv mutant organs contain three to four Su(H)⁺ cells (arrowheads). (D) PCR tests demonstrated that the available insv stocks are actually doubly mutant for lgl (data not shown). Expression of UAS-H2B-RFP in lgl-insv double mutant MARCM clones does not affect the multiple socket phenotype (arrowheads). H2B-RFP is green, and cuticular structures including socket and shaft cells are red. (E) Expression of UAS-lgl-GFP (green) fully rescues the bristle phenotypes of lgl-insv double mutant clones with single sockets (arrowheads) and single shafts (arrows).