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Methods Mol Biol. 2009;534:283-91. doi: 10.1007/978-1-59745-022-5_20.

Preparation of a glycan library using a variety of glycosyltrasferases.

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  • 1Research Center for Glycoscience, National Institute of Advanced Industrial Science and Technology (AIST), Open Space Laboratory C-2, 1-1-1 Umezono, Tsukuba, Ibaraki, 305-8568, Japan.

Abstract

We have comprehensively cloned genes associated with the synthesis of glycans in the human body, then translated those glycogenes into recombinant glycosyltransferases using a variety of expression systems. The library of glycans or glycopeptides having various structures can be synthesized in a test tube using various enzymes. Using the glycan library, we have recently constructed a multistage tandem mass (MS( n )) spectral library containing observed spectra. This library may be utilized as a novel tool for glycomics research, as it enables users to identify oligosaccharides very easily and quickly by spectral matching.

PMID:
19277544
[PubMed - indexed for MEDLINE]
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