The Mei5-Sae3 protein complex mediates Dmc1 activity in Saccharomyces cerevisiae

J Biol Chem. 2009 May 1;284(18):11766-70. doi: 10.1074/jbc.C900023200. Epub 2009 Mar 7.

Abstract

During homologous recombination, a number of proteins cooperate to catalyze the loading of recombinases onto single-stranded DNA. Single-stranded DNA-binding proteins stimulate recombination by coating single-stranded DNA and keeping it free of secondary structure; however, in order for recombinases to load on single-stranded-DNA-binding protein-coated DNA, the activity of a class of proteins known as recombination mediators is required. Mediator proteins coordinate the handoff of single-stranded DNA from single-stranded DNA-binding protein to recombinase. Here we show that a complex of Mei5 and Sae3 from Saccharomyces cerevisiae preferentially binds single-stranded DNA and relieves the inhibition of the strand assimilation and DNA binding abilities of the meiotic recombinase Dmc1 imposed by the single-stranded DNA-binding protein replication protein A. Additionally, we demonstrate the physical interaction of Mei5-Sae3 with replication protein A. Our results, together with previous in vivo studies, indicate that Mei5-Sae3 is a mediator of Dmc1 assembly during meiotic recombination in S. cerevisiae.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Cell Cycle Proteins / genetics
  • Cell Cycle Proteins / metabolism*
  • Chromosomal Proteins, Non-Histone / genetics
  • Chromosomal Proteins, Non-Histone / metabolism*
  • DNA, Fungal / genetics
  • DNA, Fungal / metabolism
  • DNA, Single-Stranded / genetics
  • DNA, Single-Stranded / metabolism
  • DNA-Binding Proteins / genetics
  • DNA-Binding Proteins / metabolism*
  • Meiosis / physiology*
  • Multiprotein Complexes / genetics
  • Multiprotein Complexes / metabolism*
  • Recombinases / genetics
  • Recombinases / metabolism*
  • Recombination, Genetic / physiology
  • Replication Protein A / genetics
  • Replication Protein A / metabolism
  • Saccharomyces cerevisiae / genetics
  • Saccharomyces cerevisiae / metabolism*
  • Saccharomyces cerevisiae Proteins / genetics
  • Saccharomyces cerevisiae Proteins / metabolism*

Substances

  • Cell Cycle Proteins
  • Chromosomal Proteins, Non-Histone
  • DMC1 protein, S cerevisiae
  • DNA, Fungal
  • DNA, Single-Stranded
  • DNA-Binding Proteins
  • Mei5 protein, S cerevisiae
  • Multiprotein Complexes
  • Recombinases
  • Replication Protein A
  • SAE3 protein, S cerevisiae
  • Saccharomyces cerevisiae Proteins