Display Settings:

Format

Send to:

Choose Destination
See comment in PubMed Commons below
Cell. 2009 Mar 6;136(5):852-64. doi: 10.1016/j.cell.2009.01.017.

Establishing the program of origin firing during S phase in fission Yeast.

Author information

  • 1The Rockefeller University, New York, NY 10065, USA. pwu@rockefeller.edu

Abstract

Initiation of eukaryotic DNA synthesis occurs at origins of replication that are utilized with characteristic times and frequencies during S phase. We have investigated origin usage by evaluating the kinetics of replication factor binding in fission yeast and show that similar to metazoa, ORC binding is periodic during the cell cycle, increasing during mitosis and peaking at M/G1. At an origin, the timing of ORC binding in M and pre-RC assembly in G1 correlates with the timing of firing during S, and the level of pre-IC formation reflects origin efficiency. Extending mitosis allows ORC to become more equally associated with origins and leads to genome-wide changes in origin usage, while overproduction of pre-IC factors increases replication of both efficient and inefficient origins. We propose that differential recruitment of ORC to origins during mitosis followed by competition among origins for limiting replication factors establishes the timing and efficiency of origin firing.

Comment in

PMID:
19269364
[PubMed - indexed for MEDLINE]
PMCID:
PMC2787407
Free PMC Article

Images from this publication.See all images (7)Free text

Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Elsevier Science Icon for PubMed Central Icon for Faculty of 1000
    Loading ...
    Write to the Help Desk