Biochem Biophys Res Commun. 2009 May 1;382(2):232-5. Epub 2009 Mar 5.

Production of a reporter transgenic pig for monitoring Cre recombinase activity.

Li L, Pang D, Wang T, Li Z, Chen L, Zhang M, Song N, Nie D, Chen Z, Lai L, Ouyang H.

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College of Animal Science and Veterinary Medicine, Jilin University, Changchun, PR China. lilitonghappy@hotmail.com

Abstract

The pig is thought to be the most suitable non-human source of organs for xenotransplantation and is widely used as a model of human disease. Using pigs as disease models requires the design of conditional Cre recombinase-loxP gene modifications, which, in turn, requires a Cre-expressing pig with defined patterns of expression controlled by the use of a tissue-specific promoter. In order to monitor Cre recombinant expression in vivo, it is important to create a reporter strain. We have generated reporter a pig that is based on a single vector that drives the ubiquitous expression of the enhanced green fluorescent protein (EGFP). The EGFP gene is expressed only after Cre-mediated excision of loxP-flanked stop sequences. These reporter transgenic pigs will be of great value for monitoring Cre recombinase activity in vivo.

PMID:: 19268654; [PubMed - indexed for MEDLINE]

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