Objective and design: To investigate the modulating role of lymphocytes in leukocyte recruitment in a murine model of peritonitis.
Materials or subjects: RAG-1 knockout (KO) mice, NUDE mice and microMT KO mice were compared to their wild-type controls.
Treatment: Mice were administered with 1 ml of Brewer's thioglycollate (BTG) and terminal peritoneal lavages were performed at 8, 24, 72 and 120 h after treatment.
Methods: Leukocyte numbers recruited at the different time points following a BTG administration were determined. Chemokine and cytokine levels were assessed by either ELISAs or cytometric bead array.
Results: RAG-1 KO mice (absent B and T cells) exhibited increased early neutrophil infiltration and blunted late monocyte/macrophage infiltration. NUDE mice (absent T cells) exhibited both increased neutrophil and monocyte/macrophage infiltration. In contrast, microMT KO mice (absent B cells) demonstrated reduced influx of both neutrophils and monocyte/macrophages. Chemokine analysis revealed various differences in important chemokines.
Conclusions: These data suggest that T cells act to suppress leukocyte recruitment while B cells promote leukocyte recruitment.