J Clin Microbiol. 2009 May;47(5):1562-4. doi: 10.1128/JCM.00124-09. Epub 2009 Mar 4.

Interlaboratory reproducibility of a single-locus sequence-based method for strain typing of Aspergillus fumigatus.

Hurst SF, Kidd SE, Morrissey CO, Snelders E, Melchers WJ, Castelli MV, Mellado E, Simmon K, Petti CA, Richardson S, Zhang S, Romanelli AM, Wickes BL, de Valk HA, Klaassen CH, Balajee SA.

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Mycotic Diseases Branch, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA.

Abstract

Seven international laboratories tested the recently proposed single-locus typing strategy for Aspergillus fumigatus subtyping for interlaboratory reproducibility. Comparative sequence analyses of portions of the locus AFUA_3G08990, encoding a putative cell surface protein (denoted CSP), was performed with a panel of Aspergillus isolates. Each laboratory followed very different protocols for extraction of DNA, PCR, and sequencing. Results revealed that the CSP typing method was a reproducible and portable strain typing method.

PMID:: 19261791; [PubMed - indexed for MEDLINE]
PMCID:: PMC2681842

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Interlaboratory reproducibility of a single-locus sequence-based method for stra...
Interlaboratory reproducibility of a single-locus sequence-based method for strain typing of Aspergillus fumigatus.
J Clin Microbiol. 2009 May ;47(5):1562-4. doi: 10.1128/JCM.00124-09. Epub 2009 Mar 4 .

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