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J Clin Microbiol. 2009 May;47(5):1562-4. doi: 10.1128/JCM.00124-09. Epub 2009 Mar 4.

Interlaboratory reproducibility of a single-locus sequence-based method for strain typing of Aspergillus fumigatus.

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  • 1Mycotic Diseases Branch, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA.


Seven international laboratories tested the recently proposed single-locus typing strategy for Aspergillus fumigatus subtyping for interlaboratory reproducibility. Comparative sequence analyses of portions of the locus AFUA_3G08990, encoding a putative cell surface protein (denoted CSP), was performed with a panel of Aspergillus isolates. Each laboratory followed very different protocols for extraction of DNA, PCR, and sequencing. Results revealed that the CSP typing method was a reproducible and portable strain typing method.

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