Abstract
Problem:
We previously reported that lipopolysaccharide (LPS)-promoted endometriotic stromal cell (ESC) proliferation by inducing TNFalpha production. The aim of this study was to investigate the efficacy of TNFalpha gene silencing on LPS-treated ESCs.
Method of study:
Endometriotic stromal cells (ESCs) and endometrial stromal cells (ESCs) (EMSCs) were obtained from ovarian chocolate cysts and uterine myoma, respectively. Using PCR array, LPS-induced gene expression profiling after transfection of TNFalpha siRNA into ESCs was performed. Down-regulated genes by TNFalpha silencing were examined using real-time RT-PCR. Effect of TNFalpha silencing was examined using ELISA and BrdU incorporation, respectively.
Results:
In PCR array, TNFalpha silencing in ESCs repressed LPS-induced expression of cIAP2 and IL-8, NFkappaB pathway responsive genes. After adding LPS, the levels of cIAP2 and IL-8 expression in ESCs were higher compared with those in EMSCs. TNFalpha silencing attenuated the LPS-induced ESC proliferation.
Conclusion:
Tumor necrosis factor alpha may be involved in cell proliferation of endometriotic tissues.
MeSH terms
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Apoptosis / immunology
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Baculoviral IAP Repeat-Containing 3 Protein
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Cell Proliferation / drug effects
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Cells, Cultured
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Endometriosis / genetics
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Endometriosis / immunology*
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Endometriosis / pathology
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Endometrium / pathology
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Female
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Gene Expression Profiling
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Gene Silencing
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Humans
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Inhibitor of Apoptosis Proteins / genetics
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Inhibitor of Apoptosis Proteins / immunology
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Inhibitor of Apoptosis Proteins / metabolism
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Interleukin-8 / genetics
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Interleukin-8 / immunology
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Interleukin-8 / metabolism
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Leiomyoma / genetics
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Leiomyoma / immunology*
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Leiomyoma / pathology
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Lipopolysaccharides / pharmacology
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NF-kappa B / immunology
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Ovarian Cysts / genetics
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Ovarian Cysts / immunology*
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Ovarian Cysts / pathology
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Ovarian Diseases / genetics
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Ovarian Diseases / immunology*
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Ovarian Diseases / pathology
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RNA, Small Interfering
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Signal Transduction / immunology
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Stromal Cells / immunology
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Stromal Cells / metabolism
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Stromal Cells / pathology
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Tumor Necrosis Factor-alpha / genetics*
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Tumor Necrosis Factor-alpha / immunology
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Ubiquitin-Protein Ligases
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Uterine Neoplasms / genetics
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Uterine Neoplasms / immunology*
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Uterine Neoplasms / pathology
Substances
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Inhibitor of Apoptosis Proteins
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Interleukin-8
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Lipopolysaccharides
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NF-kappa B
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RNA, Small Interfering
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Tumor Necrosis Factor-alpha
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BIRC3 protein, human
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Baculoviral IAP Repeat-Containing 3 Protein
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Ubiquitin-Protein Ligases