TNFalpha gene silencing reduced lipopolysaccharide-promoted proliferation of endometriotic stromal cells

Am J Reprod Immunol. 2009 Apr;61(4):277-85. doi: 10.1111/j.1600-0897.2009.00691.x.

Abstract

Problem: We previously reported that lipopolysaccharide (LPS)-promoted endometriotic stromal cell (ESC) proliferation by inducing TNFalpha production. The aim of this study was to investigate the efficacy of TNFalpha gene silencing on LPS-treated ESCs.

Method of study: Endometriotic stromal cells (ESCs) and endometrial stromal cells (ESCs) (EMSCs) were obtained from ovarian chocolate cysts and uterine myoma, respectively. Using PCR array, LPS-induced gene expression profiling after transfection of TNFalpha siRNA into ESCs was performed. Down-regulated genes by TNFalpha silencing were examined using real-time RT-PCR. Effect of TNFalpha silencing was examined using ELISA and BrdU incorporation, respectively.

Results: In PCR array, TNFalpha silencing in ESCs repressed LPS-induced expression of cIAP2 and IL-8, NFkappaB pathway responsive genes. After adding LPS, the levels of cIAP2 and IL-8 expression in ESCs were higher compared with those in EMSCs. TNFalpha silencing attenuated the LPS-induced ESC proliferation.

Conclusion: Tumor necrosis factor alpha may be involved in cell proliferation of endometriotic tissues.

MeSH terms

  • Apoptosis / immunology
  • Baculoviral IAP Repeat-Containing 3 Protein
  • Cell Proliferation / drug effects
  • Cells, Cultured
  • Endometriosis / genetics
  • Endometriosis / immunology*
  • Endometriosis / pathology
  • Endometrium / pathology
  • Female
  • Gene Expression Profiling
  • Gene Silencing
  • Humans
  • Inhibitor of Apoptosis Proteins / genetics
  • Inhibitor of Apoptosis Proteins / immunology
  • Inhibitor of Apoptosis Proteins / metabolism
  • Interleukin-8 / genetics
  • Interleukin-8 / immunology
  • Interleukin-8 / metabolism
  • Leiomyoma / genetics
  • Leiomyoma / immunology*
  • Leiomyoma / pathology
  • Lipopolysaccharides / pharmacology
  • NF-kappa B / immunology
  • Ovarian Cysts / genetics
  • Ovarian Cysts / immunology*
  • Ovarian Cysts / pathology
  • Ovarian Diseases / genetics
  • Ovarian Diseases / immunology*
  • Ovarian Diseases / pathology
  • RNA, Small Interfering
  • Signal Transduction / immunology
  • Stromal Cells / immunology
  • Stromal Cells / metabolism
  • Stromal Cells / pathology
  • Tumor Necrosis Factor-alpha / genetics*
  • Tumor Necrosis Factor-alpha / immunology
  • Ubiquitin-Protein Ligases
  • Uterine Neoplasms / genetics
  • Uterine Neoplasms / immunology*
  • Uterine Neoplasms / pathology

Substances

  • Inhibitor of Apoptosis Proteins
  • Interleukin-8
  • Lipopolysaccharides
  • NF-kappa B
  • RNA, Small Interfering
  • Tumor Necrosis Factor-alpha
  • BIRC3 protein, human
  • Baculoviral IAP Repeat-Containing 3 Protein
  • Ubiquitin-Protein Ligases