Two Cyclic Dipeptides from Pseudomonas fluorescens GcM5-1A Carried by the Pine Wood Nematode and Their Toxicities to Japanese Black Pine Suspension Cells and Seedlings in vitro

Qunqun Guo; Daosen Guo; Boguang Zhao; Jie Xu; Ronggui Li

Two Cyclic Dipeptides from Pseudomonas fluorescens GcM5-1A Carried by the Pine Wood Nematode and Their Toxicities to Japanese Black Pine Suspension Cells and Seedlings in vitro

J Nematol. 2007 Sep;39(3):243-7.

Authors

Qunqun Guo¹, Daosen Guo, Boguang Zhao, Jie Xu, Ronggui Li

Affiliation

¹ Department of Biology, Qingdao University, Qingdao 266071, P. R. China Department of Forest Entomology and Pathology, Nanjing Forest University, Nanjing 210037, P. R. China.

PMID: 19259494
PMCID: PMC2586505

Abstract

Pseudomonas fluorescens GcM5-1A, isolated from the pine wood nematode (PWN), Bursaphelenchus xylophilus, was cultured in Luria Broth medium (LB). The clarified culture was extracted with ethyl acetate, and two dipeptides were purified from the extract. The chemical structures of 1 and 2 were identified as cyclo(-Pro-Val-)and cyclo(-Pro-Tyr-), respectively, by MS, (1)H NMR, (13)C NMR,(1)H-(1)H COSY, 1H -(13)C COSY spectra. Bioassay results showed that the two compounds were toxic to both suspension cells and seedlings of Pinus thunbergii, which may offer some clues to research the mechanism of pine wilt disease caused by PWN.