An example of AO-OCT scanning areas used for creating larger FOV volume (based on the acquisition of nine sub-volumes). Numbers on the sub-volumes refer to the order in which they were acquired. Fundus photo is used for reference.

A simple flow chart representing the process of sub-volume co-registration and stitching.

Left, schematic of the volume manipulation allowed during manual volume stitching in our volume renderer. Right, resulting volume with common voxels.

Schematic of the manipulation allowed during co-registration of the fundus photo (green rectangle) with the OCT volume.

UHR-AO-OCT volume of the retinal structures acquired over 0.25 × 0.3 mm area on the 4.5 deg temporal retina (TR). Top row: left, visualization of the data acquired with UHR-AO-OCT system focus set on photoreceptor layers; center, fundus photo with marked location of the acquired volumes; right, visualization of the data acquired with UHR-AO-OCT system focus set on inner retinal layers. Bottom row: left, screenshot from OSA ISP with the UHR-AO-OCT volume with focus set on photoreceptor layers (View 1); right, screenshot from OSA ISP with the UHR-AO-OCT volume and focus set on inner retina layers (View 2). Multiple microscopic and cellular structures can be recognized clearly (including photoreceptors seen on the lower left panel and microcapillaries seen on the lower right panel).

OCT fundus montage created with our volume renderer of nine stitched AO-OCT 1 × 1 mm sub-volumes acquired with focus set on inner retinal layers.

Visualization of the large FOV AO-OCT volume (from Fig. 5). Left, movie of the volume with co-registered fundus photo generated with our custom visualization software (Media 1). Right, screenshot from OSA ISP with the slicing plane view of the large FOV AO-OCT volume (View 3). Note the clear visualization of microcapillaries and foveal avascular zone when altering the location of the slicing plane.

Visualization of the ONH microscopic structures imaged with AO-OCT. Left, screenshot from our custom volume renderer after co-registration of five 3D AO-OCT datasets with fundus photo. Right, screenshots from movies showing B-scan and cross-sectional view [26] of three AO-OCT volumes shown on the left: 15N 2SR (Media 2), 12NR (Media 3), and 14N 2IR (Media 4). White lines on the B-scans correspond to the depth of the C-scans reconstructed from the same volume. Multiple microscopic structures including lamina cribosa and blood vessels are easily recognizable. All the volumes can be accessed and viewed with OSA ISP: 12NR (View 4), 14N 2IR (View 5), 15N 2SR (View 6), 17N 1SR (View 7), and 16N 6SR (View 8). Note that the volume acquired at 17N 1SR has motion distortion that could not be properly corrected.