Phys Rev Lett. 2009 Feb 20;102(7):078301. Epub 2009 Feb 17.

Measurement of the torque on a single stretched and twisted DNA using magnetic tweezers.

Mosconi F, Allemand JF, Bensimon D, Croquette V.

Source

LPS-ENS, UMR 8550 CNRS, 24, rue Lhomond, 75231 Paris Cedex 05, France.

Abstract

We deduced the torque applied on a single stretched and twisted DNA by integrating the change in the molecule's extension with respect to force as it is coiled. While consistent with previous direct measurements of the torque at high forces (F>1 pN), this method, which is simple and does not require a sophisticated setup, allows for lower force estimates. We used this approach to deduce the effective torsional modulus of DNA, which decreases with force, and to estimate the buckling torque of DNA as a function of force in various salt conditions.

PMID:: 19257716; [PubMed - indexed for MEDLINE]

Publication Types, MeSH Terms, Substances

Publication Types

Research Support, Non-U.S. Gov't

MeSH Terms

Substances

LinkOut - more resources

Full Text Sources

Supplemental Content

Related citations

Magnetic torque tweezers: measuring torsional stiffness in DNA and RecA-DNA filaments. [Nat Methods. 2010]
Magnetic torque tweezers: measuring torsional stiffness in DNA and RecA-DNA filaments.
Lipfert J, Kerssemakers JW, Jager T, Dekker NH. Nat Methods. 2010 Dec; 7(12):977-80. Epub 2010 Oct 17.
Structural transitions and elasticity from torque measurements on DNA. [Nature. 2003]
Structural transitions and elasticity from torque measurements on DNA.
Bryant Z, Stone MD, Gore J, Smith SB, Cozzarelli NR, Bustamante C. Nature. 2003 Jul 17; 424(6946):338-41.
Magnetic tweezers for single-molecule manipulation. [Methods Mol Biol. 2011]
Magnetic tweezers for single-molecule manipulation.
Seol Y, Neuman KC. Methods Mol Biol. 2011; 783:265-93.
Review Structure and dynamics of single DNA molecules manipulated by magnetic tweezers and or flow. [Methods. 2009]
Review Structure and dynamics of single DNA molecules manipulated by magnetic tweezers and or flow.
Leuba SH, Wheeler TB, Cheng CM, LeDuc PR, Fernández-Sierra M, Quiñones E. Methods. 2009 Mar; 47(3):214-22. Epub 2008 Nov 17.
Review Direct measurement of torque in an optical trap and its application to double-strand DNA. [Phys Rev Lett. 2006]
Review Direct measurement of torque in an optical trap and its application to double-strand DNA.
Oroszi L, Galajda P, Kirei H, Bottka S, Ormos P. Phys Rev Lett. 2006 Aug 4; 97(5):058301. Epub 2006 Aug 1.

See reviews... See all...

Cited by 10 PubMed Central articles

Analytical description of extension, torque, and supercoiling radius of a stretched twisted DNA. [Phys Rev Lett. 2011]
Analytical description of extension, torque, and supercoiling radius of a stretched twisted DNA.
Neukirch S, Marko JF. Phys Rev Lett. 2011 Apr 1; 106(13):138104. Epub 2011 Apr 1.
Force-driven unbinding of proteins HU and Fis from DNA quantified using a thermodynamic Maxwell relation. [Nucleic Acids Res. 2011]
Force-driven unbinding of proteins HU and Fis from DNA quantified using a thermodynamic Maxwell relation.
Xiao B, Zhang H, Johnson RC, Marko JF. Nucleic Acids Res. 2011 Jul; 39(13):5568-77. Epub 2011 Mar 22.
Real-time detection of cruciform extrusion by single-molecule DNA nanomanipulation. [Nucleic Acids Res. 2011]
Real-time detection of cruciform extrusion by single-molecule DNA nanomanipulation.
Ramreddy T, Sachidanandam R, Strick TR. Nucleic Acids Res. 2011 May; 39(10):4275-83. Epub 2011 Jan 25.

See all...

Related information

Related Citations
Calculated set of PubMed citations closely related to the selected article(s) retrieved using a word weight algorithm. Related articles are displayed in ranked order from most to least relevant, with the “linked from” citation displayed first.
Compound (MeSH Keyword)
PubChem chemical compound records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.
Substance (MeSH Keyword)
PubChem chemical substance (submitted) records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.
Cited in PMC
Full-text articles in the PubMed Central Database that cite the current articles.

Recent activity

Clear Turn Off Turn On

Measurement of the torque on a single stretched and twisted DNA using magnetic t...
Measurement of the torque on a single stretched and twisted DNA using magnetic tweezers.
Phys Rev Lett. 2009 Feb 20 ;102(7):078301. Epub 2009 Feb 17 .

PubMed
Effects of pantoprazole and esomeprazole on platelet inhibition by clopidogrel.
Effects of pantoprazole and esomeprazole on platelet inhibition by clopidogrel.
Am Heart J. 2009 Jan ;157(1):148.e1-5. Epub 2008 Nov 6 .

PubMed
Measuring the value of public health systems.
Measuring the value of public health systems.
Am J Public Health. 2009 May ;99(5):775-6; author reply 776. Epub 2009 Mar 19 .

PubMed
Betaine in human nutrition.
Betaine in human nutrition.
Am J Clin Nutr. 2004 Sep ;80(3):539-49.

PubMed
Incremental value of adenosine-induced stress myocardial perfusion imaging with ...
Incremental value of adenosine-induced stress myocardial perfusion imaging with dual-source CT at cardiac CT angiography.
Radiology. 2010 Feb ;254(2):410-9.

PubMed

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

PubMed

Display Settings:

Send to: