Science. 1991 May 31;252(5010):1296-300.

Identification of the DNA binding site for NGFI-B by genetic selection in yeast.

Wilson TE, Fahrner TJ, Johnston M, Milbrandt J.

Source

Department of Pathology, Washington University School of Medicine, St. Louis, MO 63110.

Abstract

An in vivo selection system for isolating targets of DNA binding proteins in yeast was developed and used to identify the DNA binding site for the NGFI-B protein, a member of the steroid-thyroid hormone receptor superfamily. The feasibility of the technique was verified by selecting DNA fragments that contained binding sites for GCN4, a well-characterized yeast transcriptional activator. The DNA binding domain of NGFI-B, expressed as part of a LexA-NGFI-B-GAL4 chimeric activator, was then used to isolate a rat genomic DNA fragment that contained an NGFI-B binding site. The NGFI-B response element (NBRE) is similar to but functionally distinct from elements recognized by the estrogen and thyroid hormone receptors and the hormone receptor-like proteins COUP-TF, CF1, and H-2RIIBP. Cotransfection experiments in mammalian cells demonstrated that NGFI-B can activate transcription from the NBRE with or without its putative ligand binding domain.

PMID:: 1925541; [PubMed - indexed for MEDLINE]

Publication Types, MeSH Terms, Substances, Grant Support

Publication Types

MeSH Terms

Substances

Grant Support

LinkOut - more resources

Full Text Sources

Other Literature Sources

COS Scholar Universe

Molecular Biology Databases

Saccharomyces Genome Database

Supplemental Content

Save items

Related citations in PubMed

A genetic method for defining DNA-binding domains: application to the nuclear receptor NGFI-B. [Proc Natl Acad Sci U S A. 1993]
A genetic method for defining DNA-binding domains: application to the nuclear receptor NGFI-B.
Wilson TE, Padgett KA, Johnston M, Milbrandt J. Proc Natl Acad Sci U S A. 1993 Oct 1; 90(19):9186-90.
The NGFI-B gene, a transcriptionally inducible member of the steroid receptor gene superfamily: genomic structure and expression in rat brain after seizure induction. [Mol Cell Biol. 1989]
The NGFI-B gene, a transcriptionally inducible member of the steroid receptor gene superfamily: genomic structure and expression in rat brain after seizure induction.
Watson MA, Milbrandt J. Mol Cell Biol. 1989 Oct; 9(10):4213-9.
Three related brain nuclear receptors, NGFI-B, Nurr1, and NOR-1, as transcriptional activators. [J Mol Neurosci. 1995]
Three related brain nuclear receptors, NGFI-B, Nurr1, and NOR-1, as transcriptional activators.
Paulsen RF, Granas K, Johnsen H, Rolseth V, Sterri S. J Mol Neurosci. 1995; 6(4):249-55.
RNR-1, a nuclear receptor in the NGFI-B/Nur77 family that is rapidly induced in regenerating liver. [J Biol Chem. 1993]
RNR-1, a nuclear receptor in the NGFI-B/Nur77 family that is rapidly induced in regenerating liver.
Scearce LM, Laz TM, Hazel TG, Lau LF, Taub R. J Biol Chem. 1993 Apr 25; 268(12):8855-61.
Domains regulating transcriptional activity of the inducible orphan receptor NGFI-B. [J Biol Chem. 1992]
Domains regulating transcriptional activity of the inducible orphan receptor NGFI-B.
Paulsen RE, Weaver CA, Fahrner TJ, Milbrandt J. J Biol Chem. 1992 Aug 15; 267(23):16491-6.

See reviews... See all...

Cited by 94 PubMed Central articles

Nurr1 regulates Top IIβ and functions in axon genesis of mesencephalic dopaminergic neurons. [Mol Neurodegener. 2012]
Nurr1 regulates Top IIβ and functions in axon genesis of mesencephalic dopaminergic neurons.
Heng X, Jin G, Zhang X, Yang D, Zhu M, Fu S, Li X, Le W. Mol Neurodegener. 2012 Feb 2; 7:4. Epub 2012 Feb 2.
NR4A gene expression is dynamically regulated in the ventral tegmental area dopamine neurons and is related to expression of dopamine neurotransmission genes. [J Mol Neurosci. 2012]
NR4A gene expression is dynamically regulated in the ventral tegmental area dopamine neurons and is related to expression of dopamine neurotransmission genes.
Eells JB, Wilcots J, Sisk S, Guo-Ross SX. J Mol Neurosci. 2012 Mar; 46(3):545-53. Epub 2011 Sep 20.
Rasd1 modulates the coactivator function of NonO in the cyclic AMP pathway. [PLoS One. 2011]
Rasd1 modulates the coactivator function of NonO in the cyclic AMP pathway.
Ong SA, Tan JJ, Tew WL, Chen KS. PLoS One. 2011; 6(9):e24401. Epub 2011 Sep 7.

See all...

Related information

Related Citations
Calculated set of PubMed citations closely related to the selected article(s) retrieved using a word weight algorithm. Related articles are displayed in ranked order from most to least relevant, with the “linked from” citation displayed first.
Substance (MeSH Keyword)
PubChem chemical substance (submitted) records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.
Cited in PMC
Full-text articles in the PubMed Central Database that cite the current articles.

Recent activity

Clear Turn Off Turn On

Identification of the DNA binding site for NGFI-B by genetic selection in yeast.
Identification of the DNA binding site for NGFI-B by genetic selection in yeast.
Science. 1991 May 31 ;252(5010):1296-300.

PubMed
APOE genotype modulates the effect of serum calcium levels on cognitive function...
APOE genotype modulates the effect of serum calcium levels on cognitive function in old age.
Neurology. 2009 Mar 3 ;72(9):821-8.

PubMed
GLANCE: results of a phase 2, randomized, double-blind, placebo-controlled study...
GLANCE: results of a phase 2, randomized, double-blind, placebo-controlled study.
Neurology. 2009 Mar 3 ;72(9):806-12.

PubMed
Posttraumatic stress disorder and suicide attempts in a community sample of urba...
Posttraumatic stress disorder and suicide attempts in a community sample of urban american young adults.
Arch Gen Psychiatry. 2009 Mar ;66(3):305-11.

PubMed
Electronic data publishing and GenBank.
Electronic data publishing and GenBank.
Science. 1991 May 31 ;252(5010):1273-7.

PubMed

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

PubMed

Result Filters

Display Settings:

Send to: