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Biophys J. 2009 Mar 4;96(5):1866-74.

Kinetics of repeat propagation in the microgene polymerization reaction.

Itsko M, Rabinovitch A, Zaritsky A.

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Department of Life Sciences, Ben-Gurion University of the Negev, Be'er-Sheva 84105, Israel. itskom@niehs.nih.gov

Abstract

Repetitive DNA is a periodic copolymer with the intrinsic property of exponential propagation to longer repeats. Microgene polymerization reaction (MPR) is a model system in which a short nonrepetitive homo-duplex DNA evolves to multiple repetitive products during heat-cool cycles. The mechanism underlying this process involves staggered annealing of complementary DNA strands of variable lengths and polymerase-mediated filling-in of the generated overhangs. MPR is considered here as a process sharing common features with two polymerization types, chain-growth and step-growth, and significant distinctions from both types were highlighted. The involved reaction stages were formulated and a kinetic model was derived and tested experimentally. The model can quantitatively explain MPR propagation and be used as a good approximation for this phenomenon.

Comment in

Biophys J. 2009 Mar 4;96(5):1673-4.

PMID:: 19254545; [PubMed - indexed for MEDLINE]
PMCID:: PMC2717307

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