Send to

Choose Destination
See comment in PubMed Commons below
Se Pu. 2008 Nov;26(6):649-57.

[Development of hydrophilic interaction chromatographic hyphenated techniques and their applications].

[Article in Chinese]

Author information

  • 1Open Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.


Hydrophilic interaction chromatography (HILIC) is popular in the analysis of hydrophilic solutes in recent years. As a unique liquid chromatographic (LC) separation mode, it has not only been successfully utilized for the separation of target compounds from complex matrixes, but also been hyphenated with other separation modes, such as reversed-phase LC (RPLC), ion-exchange chromatography, and various HILIC, to increase the resolution and peak capacity for "entire-component" analysis of complex samples. Here we briefly review the origin and concept of HILIC, its retention behavior, and the frequently used stationary phases. After discussing the selectivity orthogonality between HILIC and RPLC, several application areas of HILIC are introduced, including HILIC tandem mass spectrometry (HILIC-MS(n)), on-line HILIC pretreatment, comprehensive two-dimensional HILIC-MS(n) and on-line hyphenation of HILIC and RPLC. With abundant references, HILIC has been proved to be a powerful analytical tool for hydrophilic and polar complexes.

[PubMed - indexed for MEDLINE]
PubMed Commons home

PubMed Commons

How to join PubMed Commons

    Supplemental Content

    Loading ...
    Write to the Help Desk