Nihon Rinsho Meneki Gakkai Kaishi. 2009 Feb;32(1):43-7.

[Analysis of autoantigens in patients with systemic lupus erythematosus by using proteomic approach].

[Article in Japanese]

Iizuka N, Okamoto K, Hirohata S, Kato T.

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Department of Clinical Proteomics & Molecular Medicine, St. Marianna University Graduate School of Medicine.

Abstract

Analysis of autoantibodies/antigens has very important impact for investigation of the pathogenesis of autoimmune diseases, including systemic lupus erythematosus (SLE). Recent progress has enabled us to detect and analyze the autoantibodies/antigens easily by using a proteomics approach. By proteomics, we can directly detect proteins as gene products as well as their alterations by post-translational modification and internal abscission which are characteristically observed in proteins. For example, we pick up autoantigens of interest as spots through combination of two-dimensional electrophoresis and western blot, and identify them by analysis using mass spectrometry. These methods allow us to use clinical specimens, including various tissues as a source of autoantigens. In this review, we introduce application of proteomics approach to autoimmune diseases, referring to our study of the autoantigens detected by anti-neuronal antibodies in SLE patients with central nervous system involvement.

PMID:: 19252377; [PubMed - indexed for MEDLINE]

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