Res Microbiol. 1991 Feb-Apr;142(2-3):169-75.

The tus gene of Escherichia coli: autoregulation, analysis of flanking sequences and identification of a complementary system in Salmonella typhimurium.

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Molecular, Cellular and Developmental Biology Department, University of Colorado, Boulder 80309.

Abstract

The tus gene of Escherichia coli encodes a DNA-binding protein that, when bound to terminator sites, blocks replication forks. One of these sites, TerB, is immediately upstream from tus, and we have determined that the 5' end of tus mRNA is in the TerB site, that tus is autoregulated and that pTus is a very low efficiency promoter. Analysis of the DNA upstream from tus and TerB indicates a set of sensor/regulator genes which are comparable to envZ/ompR. Although tus mutants exhibit no growth phenotype in laboratory conditions, Salmonella typhimurium and E. coli have nevertheless maintained similar termination systems. Sequence homology can be demonstrated by Southern hybridizations, and the systems also exhibit functional complementation: the Tus protein of S. typhimurium blocks DNA replication at the TerA site of E. coli.

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tus, the trans-acting gene required for termination of DNA replication in Escherichia coli, encodes a DNA-binding protein. [Proc Natl Acad Sci U S A. 1989]
tus, the trans-acting gene required for termination of DNA replication in Escherichia coli, encodes a DNA-binding protein.
Hill TM, Tecklenburg ML, Pelletier AJ, Kuempel PL. Proc Natl Acad Sci U S A. 1989 Mar; 86(5):1593-7.
Site-directed mutagenesis and phylogenetic comparisons of the Escherichia coli Tus protein: DNA-protein interactions alone can not account for Tus activity. [Mol Genet Genomics. 2001]
Site-directed mutagenesis and phylogenetic comparisons of the Escherichia coli Tus protein: DNA-protein interactions alone can not account for Tus activity.
Henderson TA, Nilles AF, Valjavec-Gratian M, Hill TM. Mol Genet Genomics. 2001 Aug; 265(6):941-53.
In vivo characterization of tus gene expression in Escherichia coli. [Mol Microbiol. 1992]
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Roecklein BA, Kuempel PL. Mol Microbiol. 1992 Jun; 6(12):1655-61.
Review Replication termination in Escherichia coli: structure and antihelicase activity of the Tus-Ter complex. [Microbiol Mol Biol Rev. 2005]
Review Replication termination in Escherichia coli: structure and antihelicase activity of the Tus-Ter complex.
Neylon C, Kralicek AV, Hill TM, Dixon NE. Microbiol Mol Biol Rev. 2005 Sep; 69(3):501-26.
Review FNR and its role in oxygen-regulated gene expression in Escherichia coli. [FEMS Microbiol Rev. 1990]
Review FNR and its role in oxygen-regulated gene expression in Escherichia coli.
Spiro S, Guest JR. FEMS Microbiol Rev. 1990 Aug; 6(4):399-428.

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Cited by 3 PubMed Central articles

Replication termination mechanism as revealed by Tus-mediated polar arrest of a sliding helicase. [Proc Natl Acad Sci U S A. 2008]
Replication termination mechanism as revealed by Tus-mediated polar arrest of a sliding helicase.
Bastia D, Zzaman S, Krings G, Saxena M, Peng X, Greenberg MM. Proc Natl Acad Sci U S A. 2008 Sep 2; 105(35):12831-6. Epub 2008 Aug 15.
Review Replication termination in Escherichia coli: structure and antihelicase activity of the Tus-Ter complex. [Microbiol Mol Biol Rev. 2005]
Review Replication termination in Escherichia coli: structure and antihelicase activity of the Tus-Ter complex.
Neylon C, Kralicek AV, Hill TM, Dixon NE. Microbiol Mol Biol Rev. 2005 Sep; 69(3):501-26.
TerF, the sixth identified replication arrest site in Escherichia coli, is located within the rcsC gene. [J Bacteriol. 1992]
TerF, the sixth identified replication arrest site in Escherichia coli, is located within the rcsC gene.
Sharma B, Hill TM. J Bacteriol. 1992 Dec; 174(23):7854-8.

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