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Anal Bioanal Chem. 2009 Apr;393(8):1943-8. doi: 10.1007/s00216-009-2687-y. Epub 2009 Feb 24.

Aptamer-based electrochemical biosensor for Botulinum neurotoxin.

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  • 1Department of Mechanical and Aerospace Engineering, School of Engineering and Applied Science, University of California at Los Angeles, 420 Westwood Plaza, Los Angeles, CA 90095-1597, USA.


We have developed an aptamer-based electrochemical sensor for detection of Botulinum neurotoxin, where steric hindrance is applied to achieve specific signal amplification via conformational change of the aptamer. The incubation time and potassium concentration of the reaction buffer were found to be key parameters affecting the sensitivity of detection of the recognition of Botulinum neurotoxin by the aptamer. Under optimized experimental conditions, a high signal-to-noise ratio was obtained within 24 h with a limit of detection (LOD) of 40 pg/ml by two standard deviation cutoffs above the noise level.

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