(A) Correlation of Mrp score with the Gleason score of prostate cancer tissues. Each circle represent a tumor tissue in the arbitrarily grouped Gleason score. (B) GPSM score of the tumor tissues in nonrecurrence and recurrence tumors. (C) Relationship of the Mrp score with GPSM score. Horizontal bars represent the mean in each group. n = number of samples. Mean and standard deviation are shown in parentheses. *p <.05. (D) ROC curves for prediction of recurrence with GPSM score, preoperative PSA, Mrp score, and the combination. GPSM score: Sensitivity = 63.3%, specificity = 78.1% (AUC = 0.755). Preoperative PSA: Sensitivity = 66.7%, specificity = 65.6% (AUC = 0.686). Mrp score: Sensitivity = 71.0%, specificity = 71 9% (AUC = 0.773). Combined: Sensitivity = 80.0%, specificity = 81 2% (AUC = 0.852).

(A) Cluster diagram depicting CpG units methylation of genes in benign (n = 32) and tumor (n = 64) tissues. Each row represents a tissue and each column a CpG unit of the gene. Frequency of methylation levels is color scaled from red to yellow. Red denotes no methylation, while bright yellow denotes complete methylation. Gray represents technically inadequate or missing data. (B) Group mean methylation of the CpG unit of genes in the benign and tumor tissues. (C) Distribution of methylation similarity scores for diagnosis of prostate cancer (Mdp) in benign (black bars) and tumor tissues (white bars). (D) Mdp score in benign and tumor tissues with preoperative serum PSA value of <10 ng/mL and >10 ng/mL groups. n = number of tissues in each group. Mean and standard deviation are shown in parentheses. *p < .05.

(A) Cluster diagram depicting CpG units methylation of genes in nonrecurrence and recurrence tumor tissues. Each row represents a tissue and each column a CpG unit of the genes. Green represents nonrecurrent (n = 32) tumors. Blue, yellow, and red represents recurrent tumors (n = 32 total) with PSA failure (n = 10), local recurrence (n = 10), and systemic progression (n = 12), respectively. Frequency of methylation levels is color scaled from red to yellow. Red denotes no methylation, while bright yellow denotes complete methylation. Gray represents technically inadequate or missing data. (B) Group mean methylation of the CpG units of genes in nonrecurrence and recurrence tumors (p value < 0.05). (C) Distribution of methylation similarity scores for recurrence of prostate cancer (Mrp) in nonrecurrence (black bars) and recurrence (white bars) tumor tissues. (D) Mrp score in nonrecurrent and recurrent tumors with PSA failure, local, and systemic progression. n = number of tissues in each group. Mean and standard deviation are shown in parentheses. *p < .05.