Send to:

Choose Destination
See comment in PubMed Commons below
J Proteome Res. 2009 Mar;8(3):1131-42. doi: 10.1021/pr8005606.

Transcriptome and proteome analysis of osteocytes treated with nitrogen-containing bisphosphonates.

Author information

  • 1Department of Biomedical Sciences and Technologies, University of Udine, Udine, Italy, Shimadzu Research Laboratory (Europe), Manchester, UK.


We combined high-throughput screening of differential mRNAs with mass spectrometric characterization of proteins obtained from osteocytes untreated and treated with Risedronate. Microarray analysis revealed, upon treatment, a marked upregulation of messengers encoding zinc-proteins. MS analysis identified 84 proteins in the osteocytes proteome map. Risedronate affected the expression of 10 proteins, associated with cytoskeleton, stress-response and metabolism. Data validated using gel imaging in combination with the GLaD post digestion isotopic labeling method provide the molecular basis for understanding the role of bisphosphonates as antiapoptotic drugs for osteocytes.

[PubMed - indexed for MEDLINE]
PubMed Commons home

PubMed Commons

How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for American Chemical Society
    Loading ...
    Write to the Help Desk