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J Microsc. 2009 Feb;233(2):244-50. doi: 10.1111/j.1365-2818.2009.03114.x.

Chemically specific imaging of cryptosporidium oocysts using coherent anti-Stokes Raman scattering (CARS) microscopy.

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  • 1School of Information Technology and Engineering (SITE), University of Ottawa, 800 King Edward, P.O. Box 450, Stn A, Ottawa, Ontario K1N6N5, Canada. murugkar@site.uottawa.ca


We demonstrate the application of coherent anti-Stokes Raman scattering microscopy for the rapid, label-free chemical imaging of waterborne pathogens. Chemically selective images of cryptosporidium were acquired in just a few seconds using coherent anti-Stokes Raman scattering microscopy, demonstrating its capability for the rapid detection of cryptosporidium at the single oocyst level. We discuss the applicability of such a technique in a near-real time automated water testing system.

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