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Mol Cell Biol. 1991 Oct;11(10):5229-43.

LyF-1, a transcriptional regulator that interacts with a novel class of promoters for lymphocyte-specific genes.

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  • 1Howard Hughes Medical Institute, Molecular Biology Institute, UCLA School of Medicine 90024.

Abstract

We have studied transcriptional control of the murine terminal deoxynucleotidyltransferase (TdT) gene, which is activated specifically in immature B and T lymphocytes. This analysis has led to the identification and purification of a 50-kDa sequence-specific DNA-binding protein, LyF-1, that interacts with the approximate consensus sequence PyPyTGGGAGPu and is enriched in cells at most stages of B- and T-cell differentiation. LyF-1 binds tightly to an element in the TdT promoter that we show is required for transcription in lymphocytes. LyF-1 also interacts with an element in the immunoglobulin mu enhancer, called microB, that was recently shown to be important for lymphocyte-specific enhancer activity. Moreover, LyF-1 binds to the promoters for the lymphocyte-specific genes lambda 5, VpreB, and lck, all of which we speculate have additional features in common with the TdT promoter. Thus, LyF-1 may be a general transcriptional activator for genes whose expression is restricted to the B- and/or T-lymphocyte lineages.

PMID:
1922043
PMCID:
PMC361569
[PubMed - indexed for MEDLINE]
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