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Appl Environ Microbiol. 2009 Apr;75(8):2566-72. doi: 10.1128/AEM.02260-08. Epub 2009 Feb 13.

Effect of biowaste sludge maturation on the diversity of thermophilic bacteria and archaea in an anaerobic reactor.

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  • 1Universität Innsbruck, Institut für Mikrobiologie, Technikerstrasse 25 d, 6020, Innsbruck, Austria.


Prokaryotic diversity was investigated near the inlet and outlet of a plug-flow reactor. After analyzing 800 clones, 50 bacterial and 3 archaeal phylogenetic groups were defined. Clostridia (>92%) dominated among bacteria and Methanoculleus (>90%) among archaea. Significant changes in pH and volatile fatty acids did not invoke a major shift in the phylogenetic groups. We suggest that the environmental filter imposed by the saline conditions (20 g liter(-1)) selected a stable community of halotolerant and halophilic prokaryotes.

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